DNA-directed RNA polymerases I, II, and III subunit RPABC5
Intermolecular
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 7
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 10
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 45
Intramolecular
Cysteine 7 and cysteine 10
Cysteine 7 and cysteine 45
Cysteine 7 and cysteine 44
Cysteine 10 and cysteine 45
Cysteine 44 and cysteine 45
Cysteine 10 and cysteine 44
7nvy C 236 J 7
A redox-regulated disulphide may form between cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 7 of DNA-directed RNA polymerases I, II, and III subunit RPABC5. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
42
PDB code
7nvy
Structure name
rna polymerase ii pre-initiation complex with closed promoter dna in proximal position
Structure deposition date
2021-03-16
Thiol separation (Å)
10
Half-sphere exposure sum ?
91
Minimum pKa ?
3
% buried
100
Peptide A name
DNA-directed RNA polymerase II subunit RPB3
Peptide B name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Peptide A accession
I3LCH3
Peptide B accession
A0A4X1VYD0
Peptide A residue number
236
Peptide B residue number
7
Ligandability
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 7 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
7ol0 C 236 J 10
A redox-regulated disulphide may form between cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 10 of DNA-directed RNA polymerases I, II, and III subunit RPABC5. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
33
PDB code
7ol0
Structure name
structure of active transcription elongation complex pol ii-dsif (spt5-kow5)
Structure deposition date
2021-05-18
Thiol separation (Å)
10
Half-sphere exposure sum ?
96
Minimum pKa ?
9
% buried
nan
Peptide A name
DNA-directed RNA polymerase II subunit RPB3
Peptide B name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Peptide A accession
I3LCH3
Peptide B accession
A0A4X1VYD0
Peptide A residue number
236
Peptide B residue number
10
Ligandability
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 10 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
7nvz C 236 J 45
A redox-regulated disulphide may form between cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 45 of DNA-directed RNA polymerases I, II, and III subunit RPABC5. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
28
PDB code
7nvz
Structure name
rna polymerase ii pre-initiation complex with closed promoter dna in distal position
Structure deposition date
2021-03-16
Thiol separation (Å)
10
Half-sphere exposure sum ?
87
Minimum pKa ?
10
% buried
100
Peptide A name
DNA-directed RNA polymerase II subunit RPB3
Peptide B name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Peptide A accession
I3LCH3
Peptide B accession
A0A4X1VYD0
Peptide A residue number
236
Peptide B residue number
45
Ligandability
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 45 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
7nvz J 7 J 10
A redox-regulated disulphide may form within DNA-directed RNA polymerases I, II, and III subunit RPABC5 between cysteines 7 and 10.
Details
Redox score ?
78
PDB code
7nvz
Structure name
rna polymerase ii pre-initiation complex with closed promoter dna in distal position
Structure deposition date
2021-03-16
Thiol separation (Å)
4
Half-sphere exposure sum ?
101
Minimum pKa ?
4
% buried
nan
Peptide accession
A0A4X1VYD0
Residue number A
7
Residue number B
10
Peptide name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Ligandability
Cysteine 7 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Cysteine 10 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
7nvz J 7 J 45
A redox-regulated disulphide may form within DNA-directed RNA polymerases I, II, and III subunit RPABC5 between cysteines 7 and 45.
Details
Redox score ?
78
PDB code
7nvz
Structure name
rna polymerase ii pre-initiation complex with closed promoter dna in distal position
Structure deposition date
2021-03-16
Thiol separation (Å)
4
Half-sphere exposure sum ?
89
Minimum pKa ?
4
% buried
100
Peptide accession
A0A4X1VYD0
Residue number A
7
Residue number B
45
Peptide name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Ligandability
Cysteine 7 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Cysteine 45 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
7oky J 7 J 44
A redox-regulated disulphide may form within DNA-directed RNA polymerases I, II, and III subunit RPABC5 between cysteines 7 and 44.
Details
Redox score ?
76
PDB code
7oky
Structure name
structure of active transcription elongation complex pol ii-dsif-ell2- eaf1(composite structure)
Structure deposition date
2021-05-18
Thiol separation (Å)
4
Half-sphere exposure sum ?
97
Minimum pKa ?
4
% buried
100
Peptide accession
A0A4X1VYD0
Residue number A
7
Residue number B
44
Peptide name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Ligandability
Cysteine 7 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Cysteine 44 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
7nvs J 10 J 45
A redox-regulated disulphide may form within DNA-directed RNA polymerases I, II, and III subunit RPABC5 between cysteines 10 and 45. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
59
PDB code
7nvs
Structure name
rna polymerase ii core pre-initiation complex with closed promoter dna in proximal position
Structure deposition date
2021-03-16
Thiol separation (Å)
3
Half-sphere exposure sum ?
95
Minimum pKa ?
14
% buried
nan
Peptide accession
A0A4X1VYD0
Residue number A
10
Residue number B
45
Peptide name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Ligandability
Cysteine 10 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Cysteine 45 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
7oop J 44 J 45
A redox-regulated disulphide may form within DNA-directed RNA polymerases I, II, and III subunit RPABC5 between cysteines 44 and 45. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
57
PDB code
7oop
Structure name
pol ii-csb-csa-ddb1-uvssa-paf-spt6 (structure 3)
Structure deposition date
2021-05-28
Thiol separation (Å)
4
Half-sphere exposure sum ?
90
Minimum pKa ?
14
% buried
100
Peptide accession
A0A4X1VYD0
Residue number A
44
Residue number B
45
Peptide name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Ligandability
Cysteine 44 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Cysteine 45 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
7nvz J 10 J 44
A redox-regulated disulphide may form within DNA-directed RNA polymerases I, II, and III subunit RPABC5 between cysteines 10 and 44. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
35
PDB code
7nvz
Structure name
rna polymerase ii pre-initiation complex with closed promoter dna in distal position
Structure deposition date
2021-03-16
Thiol separation (Å)
4
Half-sphere exposure sum ?
103
Minimum pKa ?
24
% buried
nan
Peptide accession
A0A4X1VYD0
Residue number A
10
Residue number B
44
Peptide name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Ligandability
Cysteine 10 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Cysteine 44 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
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