ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

nitric-oxide synthase (NADPH)

Intermolecular
Cysteine 94 and cysteine 94
Cysteine 99 and cysteine 99
Cysteine 382 and cysteine 382
Intramolecular
Cysteine 94 and cysteine 99
Cysteine 184 and cysteine 441
A redox-regulated disulphide may form between two units of nitric-oxide synthase (NADPH) at cysteines 94 and 94.

Details

Redox score ?
74
PDB code
6nh2
Structure name
structure of human endothelial nitric oxide synthase heme domain in complex with (r)-6-(3-fluoro-5-(2-(pyrrolidin-2-yl)ethyl)phenethyl)- 4-methylpyridin-2-amine
Structure deposition date
2018-12-21
Thiol separation (Å)
4
Half-sphere exposure sum ?
63
Minimum pKa ?
nan
% buried
nan
Peptide A name
nitric-oxide synthase (NADPH)
Peptide B name
nitric-oxide synthase (NADPH)
Peptide A accession
A0S0A6
Peptide B accession
A0S0A6
Peptide A residue number
94
Peptide B residue number
94

Ligandability

A redox-regulated disulphide may form between two units of nitric-oxide synthase (NADPH) at cysteines 99 and 99.

Details

Redox score ?
69
PDB code
6nh1
Structure name
structure of human endothelial nitric oxide synthase heme domain in complex with 6-(3-fluoro-5-(3-(methylamino)prop-1-yn-1-yl)phenethyl)- 4-methylpyridin-2-amine
Structure deposition date
2018-12-21
Thiol separation (Å)
4
Half-sphere exposure sum ?
82
Minimum pKa ?
nan
% buried
nan
Peptide A name
nitric-oxide synthase (NADPH)
Peptide B name
nitric-oxide synthase (NADPH)
Peptide A accession
A0S0A6
Peptide B accession
A0S0A6
Peptide A residue number
99
Peptide B residue number
99

Ligandability

A redox-regulated disulphide may form between two units of nitric-oxide synthase (NADPH) at cysteines 382 and 382. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
54
PDB code
6nh2
Structure name
structure of human endothelial nitric oxide synthase heme domain in complex with (r)-6-(3-fluoro-5-(2-(pyrrolidin-2-yl)ethyl)phenethyl)- 4-methylpyridin-2-amine
Structure deposition date
2018-12-21
Thiol separation (Å)
7
Half-sphere exposure sum ?
70
Minimum pKa ?
nan
% buried
nan
Peptide A name
nitric-oxide synthase (NADPH)
Peptide B name
nitric-oxide synthase (NADPH)
Peptide A accession
A0S0A6
Peptide B accession
A0S0A6
Peptide A residue number
382
Peptide B residue number
382

Ligandability

A redox-regulated disulphide may form within nitric-oxide synthase (NADPH) between cysteines 94 and 99.

Details

Redox score ?
74
PDB code
6nh1
Structure name
structure of human endothelial nitric oxide synthase heme domain in complex with 6-(3-fluoro-5-(3-(methylamino)prop-1-yn-1-yl)phenethyl)- 4-methylpyridin-2-amine
Structure deposition date
2018-12-21
Thiol separation (Å)
4
Half-sphere exposure sum ?
73
Minimum pKa ?
nan
% buried
nan
Peptide accession
A0S0A6
Residue number A
94
Residue number B
99
Peptide name
nitric-oxide synthase (NADPH)

Ligandability

Cysteine 94 of nitric-oxide synthase (NADPH)

Cysteine 99 of nitric-oxide synthase (NADPH)

A redox-regulated disulphide may form within nitric-oxide synthase (NADPH) between cysteines 184 and 441. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
34
PDB code
6nh4
Structure name
structure of human endothelial nitric oxide synthase heme domain in complex with 6-(3-fluoro-5-(2-((2r,4s)-4-fluoropyrrolidin-2-yl) ethyl)phenethyl)-4-methylpyridin-2-amine
Structure deposition date
2018-12-21
Thiol separation (Å)
10
Half-sphere exposure sum ?
80
Minimum pKa ?
nan
% buried
nan
Peptide accession
A0S0A6
Residue number A
184
Residue number B
441
Peptide name
nitric-oxide synthase (NADPH)

Ligandability

Cysteine 184 of nitric-oxide synthase (NADPH)

Cysteine 441 of nitric-oxide synthase (NADPH)

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