DNA-directed RNA polymerase II subunit RPB3
Intermolecular
Cysteine 236 and cysteine 7 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Cysteine 236 and cysteine 10 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Cysteine 236 and cysteine 45 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Intramolecular
Cysteine 88 and cysteine 94
Cysteine 90 and cysteine 94
Cysteine 88 and cysteine 90
Cysteine 88 and cysteine 97
Cysteine 90 and cysteine 97
Cysteine 94 and cysteine 97
7nvy C 236 J 7
A redox-regulated disulphide may form between cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 7 of DNA-directed RNA polymerases I, II, and III subunit RPABC5. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
42
PDB code
7nvy
Structure name
rna polymerase ii pre-initiation complex with closed promoter dna in proximal position
Structure deposition date
2021-03-16
Thiol separation (Å)
10
Half-sphere exposure sum ?
91
Minimum pKa ?
3
% buried
100
Peptide A name
DNA-directed RNA polymerase II subunit RPB3
Peptide B name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Peptide A accession
I3LCH3
Peptide B accession
A0A4X1VYD0
Peptide A residue number
236
Peptide B residue number
7
Ligandability
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 7 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
7ol0 C 236 J 10
A redox-regulated disulphide may form between cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 10 of DNA-directed RNA polymerases I, II, and III subunit RPABC5. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
33
PDB code
7ol0
Structure name
structure of active transcription elongation complex pol ii-dsif (spt5-kow5)
Structure deposition date
2021-05-18
Thiol separation (Å)
10
Half-sphere exposure sum ?
96
Minimum pKa ?
9
% buried
nan
Peptide A name
DNA-directed RNA polymerase II subunit RPB3
Peptide B name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Peptide A accession
I3LCH3
Peptide B accession
A0A4X1VYD0
Peptide A residue number
236
Peptide B residue number
10
Ligandability
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 10 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
7nvz C 236 J 45
A redox-regulated disulphide may form between cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 45 of DNA-directed RNA polymerases I, II, and III subunit RPABC5. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
28
PDB code
7nvz
Structure name
rna polymerase ii pre-initiation complex with closed promoter dna in distal position
Structure deposition date
2021-03-16
Thiol separation (Å)
10
Half-sphere exposure sum ?
87
Minimum pKa ?
10
% buried
100
Peptide A name
DNA-directed RNA polymerase II subunit RPB3
Peptide B name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Peptide A accession
I3LCH3
Peptide B accession
A0A4X1VYD0
Peptide A residue number
236
Peptide B residue number
45
Ligandability
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 45 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
7opc C 88 C 94
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 88 and 94.
Details
Redox score ?
92
PDB code
7opc
Structure name
pol ii-csb-crl4csa-uvssa-spt6-paf (structure 4)
Structure deposition date
2021-05-31
Thiol separation (Å)
3
Half-sphere exposure sum ?
40
Minimum pKa ?
6
% buried
0
Peptide accession
I3LCH3
Residue number A
88
Residue number B
94
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 88 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 94 of DNA-directed RNA polymerase II subunit RPB3
7oo3 C 90 C 94
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 90 and 94.
Details
Redox score ?
89
PDB code
7oo3
Structure name
pol ii-csb-csa-ddb1-uvssa (structure1)
Structure deposition date
2021-05-26
Thiol separation (Å)
3
Half-sphere exposure sum ?
36
Minimum pKa ?
6
% buried
0
Peptide accession
I3LCH3
Residue number A
90
Residue number B
94
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 90 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 94 of DNA-directed RNA polymerase II subunit RPB3
7b0y C 88 C 90
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 88 and 90.
Details
Redox score ?
88
PDB code
7b0y
Structure name
structure of a transcribing rna polymerase ii-u1 snrnp complex
Structure deposition date
2020-11-23
Thiol separation (Å)
3
Half-sphere exposure sum ?
33
Minimum pKa ?
8
% buried
0
Peptide accession
I3LCH3
Residue number A
88
Residue number B
90
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 88 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 90 of DNA-directed RNA polymerase II subunit RPB3
7okx C 88 C 97
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 88 and 97.
Details
Redox score ?
84
PDB code
7okx
Structure name
structure of active transcription elongation complex pol ii-dsif (spt5-kow5)-ell2-eaf1 (composite structure)
Structure deposition date
2021-05-18
Thiol separation (Å)
4
Half-sphere exposure sum ?
57
Minimum pKa ?
6
% buried
34
Peptide accession
I3LCH3
Residue number A
88
Residue number B
97
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 88 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 97 of DNA-directed RNA polymerase II subunit RPB3
6ted C 90 C 97
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 90 and 97.
Details
Redox score ?
75
PDB code
6ted
Structure name
structure of complete, activated transcription complex pol ii-dsif- paf-spt6 uncovers allosteric elongation activation by rtf1
Structure deposition date
2019-11-11
Thiol separation (Å)
4
Half-sphere exposure sum ?
53
Minimum pKa ?
8
% buried
32
Peptide accession
I3LCH3
Residue number A
90
Residue number B
97
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 90 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 97 of DNA-directed RNA polymerase II subunit RPB3
7f4g C 94 C 97
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 94 and 97.
Details
Redox score ?
73
PDB code
7f4g
Structure name
structure of rpap2-bound rna polymerase ii
Structure deposition date
2021-06-18
Thiol separation (Å)
3
Half-sphere exposure sum ?
52
Minimum pKa ?
10
% buried
nan
Peptide accession
I3LCH3
Residue number A
94
Residue number B
97
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 94 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 97 of DNA-directed RNA polymerase II subunit RPB3
If this tool was useful for finding a disulphide, please cite: