DNA-directed RNA polymerase subunit beta
Intermolecular
Cysteine 1430 of DNA-directed RNA polymerase II subunit RPB1 and cysteine 1281
Cysteine 1428 of DNA-directed RNA polymerase subunit and cysteine 1281
Intramolecular
Cysteine 1245 and cysteine 1248
Cysteine 1245 and cysteine 1266
Cysteine 1263 and cysteine 1266
Cysteine 1248 and cysteine 1266
Cysteine 1248 and cysteine 1263
Cysteine 1245 and cysteine 1263
Cysteine 298 and cysteine 303
Cysteine 963 and cysteine 1018
More...Cysteine 483 and cysteine 698
Cysteine 636 and cysteine 833
Cysteine 347 and cysteine 350
Cysteine 350 and cysteine 698
Cysteine 152 and cysteine 822
Cysteine 347 and cysteine 698
7oo3 A 1430 B 1155
A redox-regulated disulphide may form between cysteine 1430 of DNA-directed RNA polymerase II subunit RPB1 and cysteine 1281 of DNA-directed RNA polymerase subunit beta (1430 and 1155 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
27
PDB code
7oo3
Structure name
pol ii-csb-csa-ddb1-uvssa (structure1)
Structure deposition date
2021-05-26
Thiol separation (Å)
9
Half-sphere exposure sum ?
84
Minimum pKa ?
13
% buried
100
Peptide A name
DNA-directed RNA polymerase II subunit RPB1
Peptide B name
DNA-directed RNA polymerase subunit beta
Peptide A accession
P11414
Peptide B accession
I3LGP4
Peptide A residue number
1430
Peptide B residue number
1281
Ligandability
Cysteine 1430 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 1281 of DNA-directed RNA polymerase subunit beta
6exv A 1430 B 1155
A redox-regulated disulphide may form between cysteine 1428 of DNA-directed RNA polymerase subunit and cysteine 1281 of DNA-directed RNA polymerase subunit beta (1430 and 1155 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
27
PDB code
6exv
Structure name
structure of mammalian rna polymerase ii elongation complex inhibited by alpha-amanitin
Structure deposition date
2017-11-09
Thiol separation (Å)
10
Half-sphere exposure sum ?
73
Minimum pKa ?
12
% buried
100
Peptide A name
DNA-directed RNA polymerase subunit
Peptide B name
DNA-directed RNA polymerase subunit beta
Peptide A accession
I3LJR4
Peptide B accession
I3LGP4
Peptide A residue number
1428
Peptide B residue number
1281
Ligandability
Cysteine 1428 of DNA-directed RNA polymerase subunit
Cysteine 1281 of DNA-directed RNA polymerase subunit beta
7nvt B 1119 B 1122
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 1245 and 1248 (1119 and 1122 respectively in this structure).
Details
Redox score ?
88
PDB code
7nvt
Structure name
rna polymerase ii core pre-initiation complex with closed promoter dna in distal position
Structure deposition date
2021-03-16
Thiol separation (Å)
4
Half-sphere exposure sum ?
70
Minimum pKa ?
1
% buried
70
Peptide accession
I3LGP4
Residue number A
1245
Residue number B
1248
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 1245 of DNA-directed RNA polymerase subunit beta
Cysteine 1248 of DNA-directed RNA polymerase subunit beta
6ted B 1119 B 1140
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 1245 and 1266 (1119 and 1140 respectively in this structure).
Details
Redox score ?
86
PDB code
6ted
Structure name
structure of complete, activated transcription complex pol ii-dsif- paf-spt6 uncovers allosteric elongation activation by rtf1
Structure deposition date
2019-11-11
Thiol separation (Å)
5
Half-sphere exposure sum ?
65
Minimum pKa ?
0
% buried
44
Peptide accession
I3LGP4
Residue number A
1245
Residue number B
1266
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 1245 of DNA-directed RNA polymerase subunit beta
Cysteine 1266 of DNA-directed RNA polymerase subunit beta
7nvy B 1137 B 1140
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 1263 and 1266 (1137 and 1140 respectively in this structure).
Details
Redox score ?
79
PDB code
7nvy
Structure name
rna polymerase ii pre-initiation complex with closed promoter dna in proximal position
Structure deposition date
2021-03-16
Thiol separation (Å)
4
Half-sphere exposure sum ?
61
Minimum pKa ?
6
% buried
52
Peptide accession
I3LGP4
Residue number A
1263
Residue number B
1266
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 1263 of DNA-directed RNA polymerase subunit beta
Cysteine 1266 of DNA-directed RNA polymerase subunit beta
6ted B 1122 B 1140
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 1248 and 1266 (1122 and 1140 respectively in this structure).
Details
Redox score ?
74
PDB code
6ted
Structure name
structure of complete, activated transcription complex pol ii-dsif- paf-spt6 uncovers allosteric elongation activation by rtf1
Structure deposition date
2019-11-11
Thiol separation (Å)
3
Half-sphere exposure sum ?
63
Minimum pKa ?
8
% buried
53
Peptide accession
I3LGP4
Residue number A
1248
Residue number B
1266
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 1248 of DNA-directed RNA polymerase subunit beta
Cysteine 1266 of DNA-directed RNA polymerase subunit beta
7opc B 1122 B 1137
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 1248 and 1263 (1122 and 1137 respectively in this structure).
Details
Redox score ?
72
PDB code
7opc
Structure name
pol ii-csb-crl4csa-uvssa-spt6-paf (structure 4)
Structure deposition date
2021-05-31
Thiol separation (Å)
3
Half-sphere exposure sum ?
69
Minimum pKa ?
9
% buried
74
Peptide accession
I3LGP4
Residue number A
1248
Residue number B
1263
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 1248 of DNA-directed RNA polymerase subunit beta
Cysteine 1263 of DNA-directed RNA polymerase subunit beta
7nvs B 1119 B 1137
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 1245 and 1263 (1119 and 1137 respectively in this structure).
Details
Redox score ?
72
PDB code
7nvs
Structure name
rna polymerase ii core pre-initiation complex with closed promoter dna in proximal position
Structure deposition date
2021-03-16
Thiol separation (Å)
4
Half-sphere exposure sum ?
69
Minimum pKa ?
9
% buried
64
Peptide accession
I3LGP4
Residue number A
1245
Residue number B
1263
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 1245 of DNA-directed RNA polymerase subunit beta
Cysteine 1263 of DNA-directed RNA polymerase subunit beta
7nvs B 172 B 177
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 298 and 303 (172 and 177 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
50
PDB code
7nvs
Structure name
rna polymerase ii core pre-initiation complex with closed promoter dna in proximal position
Structure deposition date
2021-03-16
Thiol separation (Å)
8
Half-sphere exposure sum ?
60
Minimum pKa ?
10
% buried
71
Peptide accession
I3LGP4
Residue number A
298
Residue number B
303
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 298 of DNA-directed RNA polymerase subunit beta
Cysteine 303 of DNA-directed RNA polymerase subunit beta
7nvz B 837 B 892
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 963 and 1018 (837 and 892 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
43
PDB code
7nvz
Structure name
rna polymerase ii pre-initiation complex with closed promoter dna in distal position
Structure deposition date
2021-03-16
Thiol separation (Å)
9
Half-sphere exposure sum ?
62
Minimum pKa ?
9
% buried
56
Peptide accession
I3LGP4
Residue number A
963
Residue number B
1018
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 963 of DNA-directed RNA polymerase subunit beta
Cysteine 1018 of DNA-directed RNA polymerase subunit beta
7nvr B 357 B 572
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 483 and 698 (357 and 572 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
40
PDB code
7nvr
Structure name
human mediator with rna polymerase ii pre-initiation complex
Structure deposition date
2021-03-15
Thiol separation (Å)
9
Half-sphere exposure sum ?
72
Minimum pKa ?
9
% buried
82
Peptide accession
I3LGP4
Residue number A
483
Residue number B
698
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 483 of DNA-directed RNA polymerase subunit beta
Cysteine 698 of DNA-directed RNA polymerase subunit beta
7nw0 B 510 B 707
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 636 and 833 (510 and 707 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
36
PDB code
7nw0
Structure name
rna polymerase ii pre-initiation complex with open promoter dna
Structure deposition date
2021-03-16
Thiol separation (Å)
8
Half-sphere exposure sum ?
96
Minimum pKa ?
12
% buried
100
Peptide accession
I3LGP4
Residue number A
636
Residue number B
833
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 636 of DNA-directed RNA polymerase subunit beta
Cysteine 833 of DNA-directed RNA polymerase subunit beta
7nw0 B 221 B 224
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 347 and 350 (221 and 224 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
35
PDB code
7nw0
Structure name
rna polymerase ii pre-initiation complex with open promoter dna
Structure deposition date
2021-03-16
Thiol separation (Å)
9
Half-sphere exposure sum ?
74
Minimum pKa ?
10
% buried
94
Peptide accession
I3LGP4
Residue number A
347
Residue number B
350
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 347 of DNA-directed RNA polymerase subunit beta
Cysteine 350 of DNA-directed RNA polymerase subunit beta
7oky B 301 B 649
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 350 and 698 (301 and 649 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
33
PDB code
7oky
Structure name
structure of active transcription elongation complex pol ii-dsif-ell2- eaf1(composite structure)
Structure deposition date
2021-05-18
Thiol separation (Å)
9
Half-sphere exposure sum ?
72
Minimum pKa ?
10
% buried
92
Peptide accession
I3LGP4
Residue number A
350
Residue number B
698
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 350 of DNA-directed RNA polymerase subunit beta
Cysteine 698 of DNA-directed RNA polymerase subunit beta
6gml B 26 B 696
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 152 and 822 (26 and 696 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
27
PDB code
6gml
Structure name
structure of paused transcription complex pol ii-dsif-nelf
Structure deposition date
2018-05-27
Thiol separation (Å)
10
Half-sphere exposure sum ?
77
Minimum pKa ?
12
% buried
98
Peptide accession
I3LGP4
Residue number A
152
Residue number B
822
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 152 of DNA-directed RNA polymerase subunit beta
Cysteine 822 of DNA-directed RNA polymerase subunit beta
7nvs B 221 B 572
A redox-regulated disulphide may form within DNA-directed RNA polymerase subunit beta between cysteines 347 and 698 (221 and 572 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
25
PDB code
7nvs
Structure name
rna polymerase ii core pre-initiation complex with closed promoter dna in proximal position
Structure deposition date
2021-03-16
Thiol separation (Å)
10
Half-sphere exposure sum ?
85
Minimum pKa ?
11
% buried
100
Peptide accession
I3LGP4
Residue number A
347
Residue number B
698
Peptide name
DNA-directed RNA polymerase subunit beta
Ligandability
Cysteine 347 of DNA-directed RNA polymerase subunit beta
Cysteine 698 of DNA-directed RNA polymerase subunit beta
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