Mediator of RNA polymerase II transcription subunit 14
Intermolecular
Cysteine 84 of Mediator of RNA polymerase II transcription subunit 10 and cysteine 115
Intramolecular
Cysteine 446 and cysteine 451
Cysteine 301 and cysteine 304
Cysteine 909 and cysteine 1209
Cysteine 295 and cysteine 300
Cysteine 304 and cysteine 306
7emf J 84 N 115
A redox-regulated disulphide may form between cysteine 84 of Mediator of RNA polymerase II transcription subunit 10 and cysteine 115 of Mediator of RNA polymerase II transcription subunit 14.
Details
Redox score ?
60
PDB code
7emf
Structure name
human mediator (deletion of med1-idr) in a tail-extended conformation
Structure deposition date
2021-04-13
Thiol separation (Å)
6
Half-sphere exposure sum ?
69
Minimum pKa ?
10
% buried
56
Peptide A name
Mediator of RNA polymerase II transcription subunit 10
Peptide B name
Mediator of RNA polymerase II transcription subunit 14
Peptide A accession
Q9BTT4
Peptide B accession
O60244
Peptide A residue number
84
Peptide B residue number
115
Ligandability
Cysteine 84 of Mediator of RNA polymerase II transcription subunit 10
Cysteine 115 of Mediator of RNA polymerase II transcription subunit 14
7enj N 446 N 451
A redox-regulated disulphide may form within Mediator of RNA polymerase II transcription subunit 14 between cysteines 446 and 451.
Details
Redox score ?
83
PDB code
7enj
Structure name
human mediator (deletion of med1-idr) in a tail-bent conformation (med-b)
Structure deposition date
2021-04-17
Thiol separation (Å)
4
Half-sphere exposure sum ?
57
Minimum pKa ?
7
% buried
56
Peptide accession
O60244
Residue number A
446
Residue number B
451
Peptide name
Mediator of RNA polymerase II transcription subunit 14
Ligandability
Cysteine 446 of Mediator of RNA polymerase II transcription subunit 14
Cysteine 451 of Mediator of RNA polymerase II transcription subunit 14
6w1s I 301 I 304
A redox-regulated disulphide may form within Mediator of RNA polymerase II transcription subunit 14 between cysteines 301 and 304. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
59
PDB code
6w1s
Structure name
atomic model of the mammalian mediator complex
Structure deposition date
2020-03-04
Thiol separation (Å)
6
Half-sphere exposure sum ?
69
Minimum pKa ?
10
% buried
24
Peptide accession
A2ABV5
Residue number A
301
Residue number B
304
Peptide name
Mediator of RNA polymerase II transcription subunit 14
Ligandability
Cysteine 301 of Mediator of RNA polymerase II transcription subunit 14
Cysteine 304 of Mediator of RNA polymerase II transcription subunit 14
6w1s I 909 I 1209
A redox-regulated disulphide may form within Mediator of RNA polymerase II transcription subunit 14 between cysteines 909 and 1209. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
42
PDB code
6w1s
Structure name
atomic model of the mammalian mediator complex
Structure deposition date
2020-03-04
Thiol separation (Å)
8
Half-sphere exposure sum ?
76
Minimum pKa ?
11
% buried
78
Peptide accession
A2ABV5
Residue number A
909
Residue number B
1209
Peptide name
Mediator of RNA polymerase II transcription subunit 14
Ligandability
Cysteine 909 of Mediator of RNA polymerase II transcription subunit 14
Cysteine 1209 of Mediator of RNA polymerase II transcription subunit 14
7nvr l 295 l 300
A redox-regulated disulphide may form within Mediator of RNA polymerase II transcription subunit 14 between cysteines 295 and 300. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
37
PDB code
7nvr
Structure name
human mediator with rna polymerase ii pre-initiation complex
Structure deposition date
2021-03-15
Thiol separation (Å)
10
Half-sphere exposure sum ?
49
Minimum pKa ?
10
% buried
52
Peptide accession
O60244
Residue number A
295
Residue number B
300
Peptide name
Mediator of RNA polymerase II transcription subunit 14
Ligandability
Cysteine 295 of Mediator of RNA polymerase II transcription subunit 14
Cysteine 300 of Mediator of RNA polymerase II transcription subunit 14
6w1s I 304 I 306
A redox-regulated disulphide may form within Mediator of RNA polymerase II transcription subunit 14 between cysteines 304 and 306. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
35
PDB code
6w1s
Structure name
atomic model of the mammalian mediator complex
Structure deposition date
2020-03-04
Thiol separation (Å)
10
Half-sphere exposure sum ?
73
Minimum pKa ?
10
% buried
36
Peptide accession
A2ABV5
Residue number A
304
Residue number B
306
Peptide name
Mediator of RNA polymerase II transcription subunit 14
Ligandability
Cysteine 304 of Mediator of RNA polymerase II transcription subunit 14
Cysteine 306 of Mediator of RNA polymerase II transcription subunit 14
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