TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L
Intermolecular
Cysteine 574 and cysteine 230 of TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L L
Cysteine 260 and cysteine 230 of TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L L
Cysteine 237 of Transcription factor SPT20 homolog and cysteine 188 L
8h7g H 574 K 230
A redox-regulated disulphide may form between cysteine 574 of TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L and cysteine 230 of TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
58
PDB code
8h7g
Structure name
cryo-em structure of the human saga complex
Structure deposition date
2022-10-20
Thiol separation (Å)
6
Half-sphere exposure sum ?
60
Minimum pKa ?
10
% buried
60
Peptide A name
TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L
Peptide B name
TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
Peptide A accession
O75529
Peptide B accession
Q9Y6J9
Peptide A residue number
574
Peptide B residue number
230
Ligandability
Cysteine 574 of TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L
Cysteine 230 of TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
8h7g H 260 K 230
A redox-regulated disulphide may form between cysteine 260 of TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L and cysteine 230 of TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
50
PDB code
8h7g
Structure name
cryo-em structure of the human saga complex
Structure deposition date
2022-10-20
Thiol separation (Å)
7
Half-sphere exposure sum ?
58
Minimum pKa ?
10
% buried
68
Peptide A name
TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L
Peptide B name
TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
Peptide A accession
O75529
Peptide B accession
Q9Y6J9
Peptide A residue number
260
Peptide B residue number
230
Ligandability
Cysteine 260 of TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L
Cysteine 230 of TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
8h7g D 237 H 188
A redox-regulated disulphide may form between cysteine 237 of Transcription factor SPT20 homolog and cysteine 188 of TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
38
PDB code
8h7g
Structure name
cryo-em structure of the human saga complex
Structure deposition date
2022-10-20
Thiol separation (Å)
9
Half-sphere exposure sum ?
60
Minimum pKa ?
11
% buried
76
Peptide A name
Transcription factor SPT20 homolog
Peptide B name
TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L
Peptide A accession
Q8NEM7
Peptide B accession
O75529
Peptide A residue number
237
Peptide B residue number
188
Ligandability
Cysteine 237 of Transcription factor SPT20 homolog
Cysteine 188 of TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L
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