ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

Ribonuclease pancreatic

Intermolecular
Cysteine 123 and cysteine 100
Cysteine 93 and cysteine 409 of Ribonuclease inhibitor
Cysteine 100 and cysteine 409 of Ribonuclease inhibitor
Intramolecular
Cysteine 93 and cysteine 100
Cysteine 68 and cysteine 123
Cysteine 54 and cysteine 112
Cysteine 86 and cysteine 138
Cysteine 100 and cysteine 138
Cysteine 136 and cysteine 118
Cysteine 84 and cysteine 118
More...
Cysteine 110 and cysteine 91
Cysteine 93 and cysteine 138
Cysteine 86 and cysteine 93
Cysteine 52 and cysteine 91
Cysteine 52 and cysteine 52
Cysteine 86 and cysteine 100
Cysteine 4 and cysteine 118
Cysteine 7 and cysteine 66
Cysteine 31 and cysteine 123
Cysteine 31 and cysteine 68
Cysteine 31 and cysteine 100
A redox-regulated disulphide may form between two units of Ribonuclease pancreatic at cysteines 123 and 100 (95 and 32 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
42
PDB code
3f8g
Structure name
the x-ray structure of a dimeric variant of human pancreatic ribonuclease with high cytotoxic and antitumor activities
Structure deposition date
2008-11-12
Thiol separation (Å)
9
Half-sphere exposure sum ?
61
Minimum pKa ?
nan
% buried
nan
Peptide A name
Ribonuclease pancreatic
Peptide B name
Ribonuclease pancreatic
Peptide A accession
P07998
Peptide B accession
P07998
Peptide A residue number
123
Peptide B residue number
100

Ligandability

Cysteine 123 of Ribonuclease pancreatic

Cysteine 100 of Ribonuclease pancreatic

Uncertain whether structure cysteine 32 has been assigned to correct residue.
A redox-regulated disulphide may form between cysteine 93 of Ribonuclease pancreatic and cysteine 409 of Ribonuclease inhibitor (65 and 408 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
36
PDB code
2q4g
Structure name
ensemble refinement of the protein crystal structure of human ribonuclease inhibitor complexed with ribonuclease i
Structure deposition date
2007-05-31
Thiol separation (Å)
9
Half-sphere exposure sum ?
65
Minimum pKa ?
10
% buried
nan
Peptide A name
Ribonuclease pancreatic
Peptide B name
Ribonuclease inhibitor
Peptide A accession
P07998
Peptide B accession
P13489
Peptide A residue number
93
Peptide B residue number
409

Ligandability

Cysteine 93 of Ribonuclease pancreatic

Cysteine 409 of Ribonuclease inhibitor

A redox-regulated disulphide may form between cysteine 100 of Ribonuclease pancreatic and cysteine 409 of Ribonuclease inhibitor (72 and 408 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
30
PDB code
2q4g
Structure name
ensemble refinement of the protein crystal structure of human ribonuclease inhibitor complexed with ribonuclease i
Structure deposition date
2007-05-31
Thiol separation (Å)
10
Half-sphere exposure sum ?
69
Minimum pKa ?
10
% buried
nan
Peptide A name
Ribonuclease pancreatic
Peptide B name
Ribonuclease inhibitor
Peptide A accession
P07998
Peptide B accession
P13489
Peptide A residue number
100
Peptide B residue number
409

Ligandability

Cysteine 100 of Ribonuclease pancreatic

Cysteine 409 of Ribonuclease inhibitor

A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 93 and 100 (165 and 172 respectively in this structure).

Details

Redox score ?
87
PDB code
1h8x
Structure name
domain-swapped dimer of a human pancreatic ribonuclease variant
Structure deposition date
2001-02-16
Thiol separation (Å)
2
Half-sphere exposure sum ?
56
Minimum pKa ?
nan
% buried
nan
Peptide accession
P07998
Residue number A
93
Residue number B
100
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 93 of Ribonuclease pancreatic

Cysteine 100 of Ribonuclease pancreatic

A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 68 and 123 (40 and 95 respectively in this structure).

Details

Redox score ?
85
PDB code
2q4g
Structure name
ensemble refinement of the protein crystal structure of human ribonuclease inhibitor complexed with ribonuclease i
Structure deposition date
2007-05-31
Thiol separation (Å)
2
Half-sphere exposure sum ?
63
Minimum pKa ?
nan
% buried
nan
Peptide accession
P07998
Residue number A
68
Residue number B
123
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 68 of Ribonuclease pancreatic

Cysteine 123 of Ribonuclease pancreatic

A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 54 and 112 (26 and 84 respectively in this structure).

Details

Redox score ?
84
PDB code
1e21
Structure name
ribonuclease 1 des1-7 crystal structure at 1
Structure deposition date
2000-05-15
Thiol separation (Å)
2
Half-sphere exposure sum ?
81
Minimum pKa ?
nan
% buried
nan
Peptide accession
P07998
Residue number A
54
Residue number B
112
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 54 of Ribonuclease pancreatic

Cysteine 112 of Ribonuclease pancreatic

A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 86 and 138 (158 and 210 respectively in this structure).

Details

Redox score ?
83
PDB code
1dza
Structure name
3-d structure of a hp-rnase
Structure deposition date
2000-02-21
Thiol separation (Å)
2
Half-sphere exposure sum ?
68
Minimum pKa ?
nan
% buried
nan
Peptide accession
P07998
Residue number A
86
Residue number B
138
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 86 of Ribonuclease pancreatic

Cysteine 138 of Ribonuclease pancreatic

A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 100 and 138 (72 and 110 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
52
PDB code
3f8g
Structure name
the x-ray structure of a dimeric variant of human pancreatic ribonuclease with high cytotoxic and antitumor activities
Structure deposition date
2008-11-12
Thiol separation (Å)
7
Half-sphere exposure sum ?
68
Minimum pKa ?
nan
% buried
nan
Peptide accession
P07998
Residue number A
100
Residue number B
138
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 100 of Ribonuclease pancreatic

Cysteine 138 of Ribonuclease pancreatic

A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 136 and 118 (110 and 118 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
50
PDB code
3ql1
Structure name
crystal structure of ribonuclease a variant a4c/d83e/v118c
Structure deposition date
2011-02-02
Thiol separation (Å)
8
Half-sphere exposure sum ?
68
Minimum pKa ?
nan
% buried
nan
Peptide accession
P61823
Residue number A
136
Residue number B
118
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 136 of Ribonuclease pancreatic

Cysteine 118 of Ribonuclease pancreatic

Cysteine 118 in protein B could not be asigned to a Uniprot residue.
A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 84 and 118 (58 and 118 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
48
PDB code
3ql1
Structure name
crystal structure of ribonuclease a variant a4c/d83e/v118c
Structure deposition date
2011-02-02
Thiol separation (Å)
8
Half-sphere exposure sum ?
67
Minimum pKa ?
nan
% buried
nan
Peptide accession
P61823
Residue number A
84
Residue number B
118
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 84 of Ribonuclease pancreatic

Cysteine 118 of Ribonuclease pancreatic

Cysteine 118 in protein B could not be asigned to a Uniprot residue.
A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 110 and 91 (84 and 85 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
47
PDB code
2op2
Structure name
crystal structure of rnase double-mutant v43c r85c with extra disulphide bond
Structure deposition date
2007-01-26
Thiol separation (Å)
8
Half-sphere exposure sum ?
67
Minimum pKa ?
nan
% buried
nan
Peptide accession
P61823
Residue number A
110
Residue number B
91
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 110 of Ribonuclease pancreatic

Cysteine 91 of Ribonuclease pancreatic

A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 93 and 138 (65 and 110 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
47
PDB code
2e0o
Structure name
mutant human ribonuclease 1 (v52l, d53l, n56l, f59l)
Structure deposition date
2006-10-10
Thiol separation (Å)
9
Half-sphere exposure sum ?
58
Minimum pKa ?
nan
% buried
nan
Peptide accession
P07998
Residue number A
93
Residue number B
138
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 93 of Ribonuclease pancreatic

Cysteine 138 of Ribonuclease pancreatic

A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 86 and 93 (58 and 65 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
45
PDB code
2k11
Structure name
solution structure of human pancreatic ribonuclease
Structure deposition date
2008-02-20
Thiol separation (Å)
9
Half-sphere exposure sum ?
53
Minimum pKa ?
nan
% buried
nan
Peptide accession
P07998
Residue number A
86
Residue number B
93
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 86 of Ribonuclease pancreatic

Cysteine 93 of Ribonuclease pancreatic

A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 52 and 91 (26 and 85 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
45
PDB code
2op2
Structure name
crystal structure of rnase double-mutant v43c r85c with extra disulphide bond
Structure deposition date
2007-01-26
Thiol separation (Å)
8
Half-sphere exposure sum ?
71
Minimum pKa ?
nan
% buried
nan
Peptide accession
P61823
Residue number A
52
Residue number B
91
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 52 of Ribonuclease pancreatic

Cysteine 91 of Ribonuclease pancreatic

A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 52 and 52 (26 and 43 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
40
PDB code
2op2
Structure name
crystal structure of rnase double-mutant v43c r85c with extra disulphide bond
Structure deposition date
2007-01-26
Thiol separation (Å)
10
Half-sphere exposure sum ?
69
Minimum pKa ?
nan
% buried
nan
Peptide accession
P61823
Residue number A
52
Residue number B
52
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 52 of Ribonuclease pancreatic

Cysteine 52 of Ribonuclease pancreatic

Uncertain whether structure cysteine 43 has been assigned to correct residue.
A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 86 and 100 (158 and 172 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
39
PDB code
1h8x
Structure name
domain-swapped dimer of a human pancreatic ribonuclease variant
Structure deposition date
2001-02-16
Thiol separation (Å)
10
Half-sphere exposure sum ?
65
Minimum pKa ?
nan
% buried
nan
Peptide accession
P07998
Residue number A
86
Residue number B
100
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 86 of Ribonuclease pancreatic

Cysteine 100 of Ribonuclease pancreatic

A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 4 and 118.

Details

Redox score ?
nan
PDB code
3ql2
Structure name
crystal structure of ribonuclease a variant a4c/d83e/v118c
Structure deposition date
2011-02-02
Thiol separation (Å)
2
Half-sphere exposure sum ?
55
Minimum pKa ?
nan
% buried
nan
Peptide accession
P61823
Residue number A
4
Residue number B
118
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 4 of Ribonuclease pancreatic

Cysteine 118 of Ribonuclease pancreatic

Cysteine 4 in protein A could not be asigned to a Uniprot residue.
Cysteine 118 in protein B could not be asigned to a Uniprot residue.
A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 7 and 66 (7 and 11 respectively in this structure).

Details

Redox score ?
nan
PDB code
4k7l
Structure name
crystal structure of rnase s variant (k7c/q11c)
Structure deposition date
2013-04-17
Thiol separation (Å)
4
Half-sphere exposure sum ?
51
Minimum pKa ?
9
% buried
32
Peptide accession
P61823
Residue number A
7
Residue number B
66
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 7 of Ribonuclease pancreatic

Cysteine 66 of Ribonuclease pancreatic

Cysteine 7 in protein A could not be asigned to a Uniprot residue.
Uncertain whether structure cysteine 11 has been assigned to correct residue.
A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 31 and 123 (31 and 95 respectively in this structure).

Details

Redox score ?
nan
PDB code
3f8g
Structure name
the x-ray structure of a dimeric variant of human pancreatic ribonuclease with high cytotoxic and antitumor activities
Structure deposition date
2008-11-12
Thiol separation (Å)
8
Half-sphere exposure sum ?
66
Minimum pKa ?
nan
% buried
nan
Peptide accession
P07998
Residue number A
31
Residue number B
123
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 31 of Ribonuclease pancreatic

Cysteine 123 of Ribonuclease pancreatic

Cysteine 31 in protein A could not be asigned to a Uniprot residue.
A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 31 and 68 (31 and 40 respectively in this structure).

Details

Redox score ?
nan
PDB code
3f8g
Structure name
the x-ray structure of a dimeric variant of human pancreatic ribonuclease with high cytotoxic and antitumor activities
Structure deposition date
2008-11-12
Thiol separation (Å)
10
Half-sphere exposure sum ?
62
Minimum pKa ?
nan
% buried
nan
Peptide accession
P07998
Residue number A
31
Residue number B
68
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 31 of Ribonuclease pancreatic

Cysteine 68 of Ribonuclease pancreatic

Cysteine 31 in protein A could not be asigned to a Uniprot residue.
A redox-regulated disulphide may form within Ribonuclease pancreatic between cysteines 31 and 100 (31 and 32 respectively in this structure).

Details

Redox score ?
nan
PDB code
3f8g
Structure name
the x-ray structure of a dimeric variant of human pancreatic ribonuclease with high cytotoxic and antitumor activities
Structure deposition date
2008-11-12
Thiol separation (Å)
7
Half-sphere exposure sum ?
62
Minimum pKa ?
nan
% buried
nan
Peptide accession
P07998
Residue number A
31
Residue number B
100
Peptide name
Ribonuclease pancreatic

Ligandability

Cysteine 31 of Ribonuclease pancreatic

Cysteine 100 of Ribonuclease pancreatic

Cysteine 31 in protein A could not be asigned to a Uniprot residue.
Uncertain whether structure cysteine 32 has been assigned to correct residue.
If this tool was useful for finding a disulphide, please cite: