ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

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DNA-directed RNA polymerases I and III subunit RPAC2

Intermolecular
Cysteine 333 of DNA-directed RNA polymerases I and III subunit RPAC1 and cysteine 107
Cysteine 565 of DNA-directed RNA polymerase III subunit RPC1 and cysteine 68
A redox-regulated disulphide may form between cysteine 333 of DNA-directed RNA polymerases I and III subunit RPAC1 and cysteine 107 of DNA-directed RNA polymerases I and III subunit RPAC2. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
54
PDB code
7a6h
Structure name
cryo-em structure of human apo rna polymerase iii
Structure deposition date
2020-08-25
Thiol separation (Å)
5
Half-sphere exposure sum ?
71
Minimum pKa ?
12
% buried
98
Peptide A name
DNA-directed RNA polymerases I and III subunit RPAC1
Peptide B name
DNA-directed RNA polymerases I and III subunit RPAC2
Peptide A accession
O15160
Peptide B accession
P0DPB6
Peptide A residue number
333
Peptide B residue number
107

Ligandability

Cysteine 333 of DNA-directed RNA polymerases I and III subunit RPAC1

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 107 of DNA-directed RNA polymerases I and III subunit RPAC2

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form between cysteine 565 of DNA-directed RNA polymerase III subunit RPC1 and cysteine 68 of DNA-directed RNA polymerases I and III subunit RPAC2. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
38
PDB code
7dn3
Structure name
structure of human rna polymerase iii elongation complex
Structure deposition date
2020-12-08
Thiol separation (Å)
8
Half-sphere exposure sum ?
89
Minimum pKa ?
12
% buried
100
Peptide A name
DNA-directed RNA polymerase III subunit RPC1
Peptide B name
DNA-directed RNA polymerases I and III subunit RPAC2
Peptide A accession
O14802
Peptide B accession
P0DPB6
Peptide A residue number
565
Peptide B residue number
68

Ligandability

Cysteine 565 of DNA-directed RNA polymerase III subunit RPC1

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 68 of DNA-directed RNA polymerases I and III subunit RPAC2

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

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