ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

Non-secretory ribonuclease

Intermolecular
Cysteine 409 of Ribonuclease inhibitor and cysteine 89
Cysteine 409 of Ribonuclease inhibitor and cysteine 98
Intramolecular
Cysteine 64 and cysteine 123
Cysteine 89 and cysteine 98
Cysteine 50 and cysteine 110
Cysteine 82 and cysteine 138
Cysteine 98 and cysteine 138
Cysteine 89 and cysteine 138
Cysteine 82 and cysteine 98
A redox-regulated disulphide may form between cysteine 409 of Ribonuclease inhibitor and cysteine 89 of Non-secretory ribonuclease (408 and 62 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
40
PDB code
2bex
Structure name
crystal structure of placental ribonuclease inhibitor in complex with human eosinophil derived neurotoxin at 2a resolution
Structure deposition date
2004-12-01
Thiol separation (Å)
9
Half-sphere exposure sum ?
71
Minimum pKa ?
11
% buried
nan
Peptide A name
Ribonuclease inhibitor
Peptide B name
Non-secretory ribonuclease
Peptide A accession
P13489
Peptide B accession
P10153
Peptide A residue number
409
Peptide B residue number
89

Ligandability

Cysteine 409 of Ribonuclease inhibitor

Cysteine 89 of Non-secretory ribonuclease

A redox-regulated disulphide may form between cysteine 409 of Ribonuclease inhibitor and cysteine 98 of Non-secretory ribonuclease (408 and 71 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
32
PDB code
2bex
Structure name
crystal structure of placental ribonuclease inhibitor in complex with human eosinophil derived neurotoxin at 2a resolution
Structure deposition date
2004-12-01
Thiol separation (Å)
10
Half-sphere exposure sum ?
77
Minimum pKa ?
11
% buried
nan
Peptide A name
Ribonuclease inhibitor
Peptide B name
Non-secretory ribonuclease
Peptide A accession
P13489
Peptide B accession
P10153
Peptide A residue number
409
Peptide B residue number
98

Ligandability

Cysteine 409 of Ribonuclease inhibitor

Cysteine 98 of Non-secretory ribonuclease

A redox-regulated disulphide may form within Non-secretory ribonuclease between cysteines 64 and 123 (37 and 96 respectively in this structure).

Details

Redox score ?
87
PDB code
1hi5
Structure name
eosinophil-derived neurotoxin (edn) - adenosine-5'-diphosphate complex
Structure deposition date
2001-01-02
Thiol separation (Å)
2
Half-sphere exposure sum ?
64
Minimum pKa ?
nan
% buried
nan
Peptide accession
P10153
Residue number A
64
Residue number B
123
Peptide name
Non-secretory ribonuclease

Ligandability

Cysteine 64 of Non-secretory ribonuclease

Cysteine 123 of Non-secretory ribonuclease

A redox-regulated disulphide may form within Non-secretory ribonuclease between cysteines 89 and 98 (62 and 71 respectively in this structure).

Details

Redox score ?
86
PDB code
2c02
Structure name
crystal structures of eosinophil-derived neurotoxin in complex with the inhibitors 5'-atp, ap3a, ap4a and ap5a
Structure deposition date
2005-08-24
Thiol separation (Å)
2
Half-sphere exposure sum ?
59
Minimum pKa ?
nan
% buried
nan
Peptide accession
P10153
Residue number A
89
Residue number B
98
Peptide name
Non-secretory ribonuclease

Ligandability

Cysteine 89 of Non-secretory ribonuclease

Cysteine 98 of Non-secretory ribonuclease

A redox-regulated disulphide may form within Non-secretory ribonuclease between cysteines 50 and 110 (23 and 83 respectively in this structure).

Details

Redox score ?
86
PDB code
6sso
Structure name
edn mutant l45h
Structure deposition date
2019-09-08
Thiol separation (Å)
2
Half-sphere exposure sum ?
71
Minimum pKa ?
nan
% buried
nan
Peptide accession
P10153
Residue number A
50
Residue number B
110
Peptide name
Non-secretory ribonuclease

Ligandability

Cysteine 50 of Non-secretory ribonuclease

Cysteine 110 of Non-secretory ribonuclease

A redox-regulated disulphide may form within Non-secretory ribonuclease between cysteines 82 and 138 (55 and 111 respectively in this structure).

Details

Redox score ?
84
PDB code
2c01
Structure name
crystal structures of eosinophil-derived neurotoxin in complex with the inhibitors 5'-atp, ap3a, ap4a and ap5a
Structure deposition date
2005-08-24
Thiol separation (Å)
2
Half-sphere exposure sum ?
72
Minimum pKa ?
nan
% buried
nan
Peptide accession
P10153
Residue number A
82
Residue number B
138
Peptide name
Non-secretory ribonuclease

Ligandability

Cysteine 82 of Non-secretory ribonuclease

Cysteine 138 of Non-secretory ribonuclease

A redox-regulated disulphide may form within Non-secretory ribonuclease between cysteines 98 and 138 (71 and 111 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
49
PDB code
1gqv
Structure name
atomic resolution (0
Structure deposition date
2001-12-05
Thiol separation (Å)
8
Half-sphere exposure sum ?
71
Minimum pKa ?
nan
% buried
nan
Peptide accession
P10153
Residue number A
98
Residue number B
138
Peptide name
Non-secretory ribonuclease

Ligandability

Cysteine 98 of Non-secretory ribonuclease

Cysteine 138 of Non-secretory ribonuclease

A redox-regulated disulphide may form within Non-secretory ribonuclease between cysteines 89 and 138 (62 and 111 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
47
PDB code
1hi3
Structure name
eosinophil-derived neurotoxin (edn) - adenosine 2'-5'-diphosphate complex
Structure deposition date
2001-01-02
Thiol separation (Å)
9
Half-sphere exposure sum ?
61
Minimum pKa ?
nan
% buried
nan
Peptide accession
P10153
Residue number A
89
Residue number B
138
Peptide name
Non-secretory ribonuclease

Ligandability

Cysteine 89 of Non-secretory ribonuclease

Cysteine 138 of Non-secretory ribonuclease

A redox-regulated disulphide may form within Non-secretory ribonuclease between cysteines 82 and 98 (55 and 71 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
39
PDB code
2bex
Structure name
crystal structure of placental ribonuclease inhibitor in complex with human eosinophil derived neurotoxin at 2a resolution
Structure deposition date
2004-12-01
Thiol separation (Å)
10
Half-sphere exposure sum ?
73
Minimum pKa ?
nan
% buried
nan
Peptide accession
P10153
Residue number A
82
Residue number B
98
Peptide name
Non-secretory ribonuclease

Ligandability

Cysteine 82 of Non-secretory ribonuclease

Cysteine 98 of Non-secretory ribonuclease

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