Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Intermolecular
Cysteine 95 of Importin-4 and cysteine 114
Intramolecular
Cysteine 381 and cysteine 387
Cysteine 381 and cysteine 464
Cysteine 387 and cysteine 464
Cysteine 261 and cysteine 276
Cysteine 419 and cysteine 464
Cysteine 430 and cysteine 453
8dyo A 95 B 114
A redox-regulated disulphide may form between cysteine 95 of Importin-4 and cysteine 114 of GTP-binding nuclear protein GSP1/CNR1. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
50
PDB code
8dyo
Structure name
cryo-em structure of importin-4 bound to rangtp
Structure deposition date
2022-08-04
Thiol separation (Å)
8
Half-sphere exposure sum ?
53
Minimum pKa ?
10
% buried
38
Peptide A name
Importin-4
Peptide B name
GTP-binding nuclear protein GSP1/CNR1
Peptide A accession
Q8TEX9
Peptide B accession
P32835
Peptide A residue number
95
Peptide B residue number
114
Ligandability
Cysteine 95 of Importin-4
Cysteine 114 of GTP-binding nuclear protein GSP1/CNR1
6xk9 A 518 A 524
A redox-regulated disulphide may form within Eukaryotic peptide chain release factor GTP-binding subunit ERF3A between cysteines 381 and 387 (518 and 524 respectively in this structure).
Details
Redox score ?
69
PDB code
6xk9
Structure name
cereblon in complex with ddb1, cc-90009, and gspt1
Structure deposition date
2020-06-25
Thiol separation (Å)
5
Half-sphere exposure sum ?
72
Minimum pKa ?
8
% buried
69
Peptide accession
P15170
Residue number A
381
Residue number B
387
Peptide name
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Ligandability
Cysteine 381 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Cysteine 387 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
6xk9 A 518 A 601
A redox-regulated disulphide may form within Eukaryotic peptide chain release factor GTP-binding subunit ERF3A between cysteines 381 and 464 (518 and 601 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
53
PDB code
6xk9
Structure name
cereblon in complex with ddb1, cc-90009, and gspt1
Structure deposition date
2020-06-25
Thiol separation (Å)
7
Half-sphere exposure sum ?
78
Minimum pKa ?
8
% buried
76
Peptide accession
P15170
Residue number A
381
Residue number B
464
Peptide name
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Ligandability
Cysteine 381 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Cysteine 464 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
3e1y H 525 H 602
A redox-regulated disulphide may form within Eukaryotic peptide chain release factor GTP-binding subunit ERF3A between cysteines 387 and 464 (525 and 602 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
53
PDB code
3e1y
Structure name
crystal structure of human erf1/erf3 complex
Structure deposition date
2008-08-05
Thiol separation (Å)
6
Half-sphere exposure sum ?
75
Minimum pKa ?
12
% buried
nan
Peptide accession
P15170
Residue number A
387
Residue number B
464
Peptide name
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Ligandability
Cysteine 387 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Cysteine 464 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
3j5y B 399 B 414
A redox-regulated disulphide may form within Eukaryotic peptide chain release factor GTP-binding subunit ERF3A between cysteines 261 and 276 (399 and 414 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
42
PDB code
3j5y
Structure name
structure of the mammalian ribosomal pre-termination complex associated with erf1-erf3-gdpnp
Structure deposition date
2013-11-21
Thiol separation (Å)
9
Half-sphere exposure sum ?
68
Minimum pKa ?
10
% buried
67
Peptide accession
P15170
Residue number A
261
Residue number B
276
Peptide name
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Ligandability
Cysteine 261 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Cysteine 276 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
3e1y G 557 G 602
A redox-regulated disulphide may form within Eukaryotic peptide chain release factor GTP-binding subunit ERF3A between cysteines 419 and 464 (557 and 602 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
40
PDB code
3e1y
Structure name
crystal structure of human erf1/erf3 complex
Structure deposition date
2008-08-05
Thiol separation (Å)
8
Half-sphere exposure sum ?
75
Minimum pKa ?
11
% buried
82
Peptide accession
P15170
Residue number A
419
Residue number B
464
Peptide name
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Ligandability
Cysteine 419 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Cysteine 464 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
6xk9 A 567 A 590
A redox-regulated disulphide may form within Eukaryotic peptide chain release factor GTP-binding subunit ERF3A between cysteines 430 and 453 (567 and 590 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
37
PDB code
6xk9
Structure name
cereblon in complex with ddb1, cc-90009, and gspt1
Structure deposition date
2020-06-25
Thiol separation (Å)
10
Half-sphere exposure sum ?
71
Minimum pKa ?
9
% buried
46
Peptide accession
P15170
Residue number A
430
Residue number B
453
Peptide name
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Ligandability
Cysteine 430 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Cysteine 453 of Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
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