DNA-directed RNA polymerase II subunit RPB3
Intermolecular
Cysteine 236 and cysteine 10 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Cysteine 236 and cysteine 7 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Cysteine 236 and cysteine 45 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
Intramolecular
Cysteine 90 and cysteine 97
Cysteine 88 and cysteine 94
Cysteine 90 and cysteine 94
Cysteine 94 and cysteine 97
Cysteine 88 and cysteine 90
Cysteine 88 and cysteine 97
6xre C 236 J 10
A redox-regulated disulphide may form between cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 10 of DNA-directed RNA polymerases I, II, and III subunit RPABC5.
Details
Redox score ?
74
PDB code
6xre
Structure name
structure of the p53/rna polymerase ii assembly
Structure deposition date
2020-07-12
Thiol separation (Å)
5
Half-sphere exposure sum ?
80
Minimum pKa ?
2
% buried
100
Peptide A name
DNA-directed RNA polymerase II subunit RPB3
Peptide B name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Peptide A accession
P19387
Peptide B accession
P62875
Peptide A residue number
236
Peptide B residue number
10
Ligandability
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 10 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
6xre C 236 J 7
A redox-regulated disulphide may form between cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 7 of DNA-directed RNA polymerases I, II, and III subunit RPABC5. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
44
PDB code
6xre
Structure name
structure of the p53/rna polymerase ii assembly
Structure deposition date
2020-07-12
Thiol separation (Å)
9
Half-sphere exposure sum ?
83
Minimum pKa ?
6
% buried
100
Peptide A name
DNA-directed RNA polymerase II subunit RPB3
Peptide B name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Peptide A accession
P19387
Peptide B accession
P62875
Peptide A residue number
236
Peptide B residue number
7
Ligandability
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 7 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
6xre C 236 J 45
A redox-regulated disulphide may form between cysteine 236 of DNA-directed RNA polymerase II subunit RPB3 and cysteine 45 of DNA-directed RNA polymerases I, II, and III subunit RPABC5. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
37
PDB code
6xre
Structure name
structure of the p53/rna polymerase ii assembly
Structure deposition date
2020-07-12
Thiol separation (Å)
8
Half-sphere exposure sum ?
83
Minimum pKa ?
12
% buried
100
Peptide A name
DNA-directed RNA polymerase II subunit RPB3
Peptide B name
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Peptide A accession
P19387
Peptide B accession
P62875
Peptide A residue number
236
Peptide B residue number
45
Ligandability
Cysteine 236 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 45 of DNA-directed RNA polymerases I, II, and III subunit RPABC5
5iy6 C 90 C 97
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 90 and 97.
Details
Redox score ?
91
PDB code
5iy6
Structure name
human holo-pic in the closed state
Structure deposition date
2016-03-24
Thiol separation (Å)
3
Half-sphere exposure sum ?
48
Minimum pKa ?
6
% buried
9
Peptide accession
P19387
Residue number A
90
Residue number B
97
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 90 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 97 of DNA-directed RNA polymerase II subunit RPB3
5flm C 88 C 94
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 88 and 94.
Details
Redox score ?
91
PDB code
5flm
Structure name
structure of transcribing mammalian rna polymerase ii
Structure deposition date
2015-10-26
Thiol separation (Å)
3
Half-sphere exposure sum ?
37
Minimum pKa ?
6
% buried
0
Peptide accession
Q3T0Q3
Residue number A
88
Residue number B
94
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 88 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 94 of DNA-directed RNA polymerase II subunit RPB3
5oik C 90 C 94
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 90 and 94.
Details
Redox score ?
86
PDB code
5oik
Structure name
structure of an rna polymerase ii-dsif transcription elongation complex
Structure deposition date
2017-07-18
Thiol separation (Å)
3
Half-sphere exposure sum ?
41
Minimum pKa ?
7
% buried
8
Peptide accession
Q3T0Q3
Residue number A
90
Residue number B
94
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 90 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 94 of DNA-directed RNA polymerase II subunit RPB3
5iy6 C 94 C 97
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 94 and 97.
Details
Redox score ?
84
PDB code
5iy6
Structure name
human holo-pic in the closed state
Structure deposition date
2016-03-24
Thiol separation (Å)
3
Half-sphere exposure sum ?
58
Minimum pKa ?
6
% buried
9
Peptide accession
P19387
Residue number A
94
Residue number B
97
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 94 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 97 of DNA-directed RNA polymerase II subunit RPB3
5oik C 88 C 90
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 88 and 90.
Details
Redox score ?
83
PDB code
5oik
Structure name
structure of an rna polymerase ii-dsif transcription elongation complex
Structure deposition date
2017-07-18
Thiol separation (Å)
3
Half-sphere exposure sum ?
45
Minimum pKa ?
9
% buried
7
Peptide accession
Q3T0Q3
Residue number A
88
Residue number B
90
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 88 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 90 of DNA-directed RNA polymerase II subunit RPB3
5flm C 88 C 97
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB3 between cysteines 88 and 97.
Details
Redox score ?
81
PDB code
5flm
Structure name
structure of transcribing mammalian rna polymerase ii
Structure deposition date
2015-10-26
Thiol separation (Å)
4
Half-sphere exposure sum ?
47
Minimum pKa ?
10
% buried
7
Peptide accession
Q3T0Q3
Residue number A
88
Residue number B
97
Peptide name
DNA-directed RNA polymerase II subunit RPB3
Ligandability
Cysteine 88 of DNA-directed RNA polymerase II subunit RPB3
Cysteine 97 of DNA-directed RNA polymerase II subunit RPB3
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