ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

Ferrochelatase, mitochondrial

Intramolecular
Cysteine 406 and cysteine 411
Cysteine 323 and cysteine 360
Cysteine 196 and cysteine 403
Cysteine 403 and cysteine 406
Cysteine 196 and cysteine 406
Cysteine 403 and cysteine 411
Cysteine 196 and cysteine 411
Cysteine 196 and cysteine 197
A redox-regulated disulphide may form within Ferrochelatase, mitochondrial between cysteines 406 and 411.

Details

Redox score ?
82
PDB code
4f4d
Structure name
f337r variant of human ferrochelatase
Structure deposition date
2012-05-10
Thiol separation (Å)
4
Half-sphere exposure sum ?
63
Minimum pKa ?
6
% buried
66
Peptide accession
P22830
Residue number A
406
Residue number B
411
Peptide name
Ferrochelatase, mitochondrial

Ligandability

Cysteine 406 of Ferrochelatase, mitochondrial

Cysteine 411 of Ferrochelatase, mitochondrial

A redox-regulated disulphide may form within Ferrochelatase, mitochondrial between cysteines 323 and 360.

Details

Redox score ?
79
PDB code
2qd1
Structure name
2
Structure deposition date
2007-06-20
Thiol separation (Å)
3
Half-sphere exposure sum ?
60
Minimum pKa ?
9
% buried
38
Peptide accession
P22830
Residue number A
323
Residue number B
360
Peptide name
Ferrochelatase, mitochondrial

Ligandability

Cysteine 323 of Ferrochelatase, mitochondrial

Cysteine 360 of Ferrochelatase, mitochondrial

A redox-regulated disulphide may form within Ferrochelatase, mitochondrial between cysteines 196 and 403.

Details

Redox score ?
72
PDB code
4klc
Structure name
e343d/f110a double mutant of human ferrochelatase
Structure deposition date
2013-05-07
Thiol separation (Å)
3
Half-sphere exposure sum ?
87
Minimum pKa ?
7
% buried
100
Peptide accession
P22830
Residue number A
196
Residue number B
403
Peptide name
Ferrochelatase, mitochondrial

Ligandability

Cysteine 196 of Ferrochelatase, mitochondrial

Cysteine 403 of Ferrochelatase, mitochondrial

A redox-regulated disulphide may form within Ferrochelatase, mitochondrial between cysteines 403 and 406 (903 and 906 respectively in this structure).

Details

Redox score ?
63
PDB code
3aqi
Structure name
h240a variant of human ferrochelatase
Structure deposition date
2010-11-03
Thiol separation (Å)
6
Half-sphere exposure sum ?
76
Minimum pKa ?
6
% buried
82
Peptide accession
P22830
Residue number A
403
Residue number B
406
Peptide name
Ferrochelatase, mitochondrial

Ligandability

Cysteine 403 of Ferrochelatase, mitochondrial

Cysteine 406 of Ferrochelatase, mitochondrial

A redox-regulated disulphide may form within Ferrochelatase, mitochondrial between cysteines 196 and 406.

Details

Redox score ?
60
PDB code
4mk4
Structure name
s197c variant of human ferrochelatase
Structure deposition date
2013-09-04
Thiol separation (Å)
7
Half-sphere exposure sum ?
68
Minimum pKa ?
6
% buried
82
Peptide accession
P22830
Residue number A
196
Residue number B
406
Peptide name
Ferrochelatase, mitochondrial

Ligandability

Cysteine 196 of Ferrochelatase, mitochondrial

Cysteine 406 of Ferrochelatase, mitochondrial

A redox-regulated disulphide may form within Ferrochelatase, mitochondrial between cysteines 403 and 411. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
53
PDB code
7ct7
Structure name
fech - inhibitor complex 2
Structure deposition date
2020-08-18
Thiol separation (Å)
7
Half-sphere exposure sum ?
81
Minimum pKa ?
7
% buried
88
Peptide accession
P22830
Residue number A
403
Residue number B
411
Peptide name
Ferrochelatase, mitochondrial

Ligandability

Cysteine 403 of Ferrochelatase, mitochondrial

Cysteine 411 of Ferrochelatase, mitochondrial

A redox-regulated disulphide may form within Ferrochelatase, mitochondrial between cysteines 196 and 411. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
46
PDB code
4mk4
Structure name
s197c variant of human ferrochelatase
Structure deposition date
2013-09-04
Thiol separation (Å)
6
Half-sphere exposure sum ?
73
Minimum pKa ?
16
% buried
88
Peptide accession
P22830
Residue number A
196
Residue number B
411
Peptide name
Ferrochelatase, mitochondrial

Ligandability

Cysteine 196 of Ferrochelatase, mitochondrial

Cysteine 411 of Ferrochelatase, mitochondrial

A redox-regulated disulphide may form within Ferrochelatase, mitochondrial between cysteines 196 and 197. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
45
PDB code
4mk4
Structure name
s197c variant of human ferrochelatase
Structure deposition date
2013-09-04
Thiol separation (Å)
7
Half-sphere exposure sum ?
74
Minimum pKa ?
12
% buried
100
Peptide accession
P22830
Residue number A
196
Residue number B
197
Peptide name
Ferrochelatase, mitochondrial

Ligandability

Cysteine 196 of Ferrochelatase, mitochondrial

Cysteine 197 of Ferrochelatase, mitochondrial

Cysteine 197 in protein B could not be asigned to a Uniprot residue.
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