DNA-directed RNA polymerase II subunit RPB1
Intermolecular
Cysteine 641 and cysteine 301 of Transcription elongation factor A protein 1
Cysteine 1430 and cysteine 1155 of DNA-directed RNA polymerase subunit beta
Cysteine 1430 and cysteine 1281 of DNA-directed RNA polymerase subunit beta
Cysteine 1430 and cysteine 1155 of DNA-directed RNA polymerase II subunit RPB2 L
Intramolecular
Cysteine 114 and cysteine 184
Cysteine 114 and cysteine 154
Cysteine 111 and cysteine 154
Cysteine 111 and cysteine 184
Cysteine 154 and cysteine 184
Cysteine 109 and cysteine 148
More...Cysteine 74 and cysteine 81
Cysteine 111 and cysteine 114
Cysteine 71 and cysteine 74
Cysteine 71 and cysteine 81
Cysteine 148 and cysteine 154
Cysteine 602 and cysteine 641
Cysteine 111 and cysteine 148
6o9l A 641 U 301
A redox-regulated disulphide may form between cysteine 641 of DNA-directed RNA polymerase II subunit RPB1 and cysteine 301 of Transcription elongation factor A protein 1.
Details
Redox score ?
67
PDB code
6o9l
Structure name
human holo-pic in the closed state
Structure deposition date
2019-03-14
Thiol separation (Å)
5
Half-sphere exposure sum ?
nan
Minimum pKa ?
9
% buried
89
Peptide A name
DNA-directed RNA polymerase II subunit RPB1
Peptide B name
Transcription elongation factor A protein 1
Peptide A accession
P24928
Peptide B accession
P23193
Peptide A residue number
641
Peptide B residue number
301
Ligandability
Cysteine 641 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 301 of Transcription elongation factor A protein 1
7b0y A 1430 B 1155
A redox-regulated disulphide may form between cysteine 1430 of DNA-directed RNA polymerase II subunit RPB1 and cysteine 1155 of DNA-directed RNA polymerase subunit beta. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
28
PDB code
7b0y
Structure name
structure of a transcribing rna polymerase ii-u1 snrnp complex
Structure deposition date
2020-11-23
Thiol separation (Å)
9
Half-sphere exposure sum ?
81
Minimum pKa ?
12
% buried
100
Peptide A name
DNA-directed RNA polymerase II subunit RPB1
Peptide B name
DNA-directed RNA polymerase subunit beta
Peptide A accession
P11414
Peptide B accession
A0A0B8RVL1
Peptide A residue number
1430
Peptide B residue number
1155
Ligandability
Cysteine 1430 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 1155 of DNA-directed RNA polymerase subunit beta
7oo3 A 1430 B 1155
A redox-regulated disulphide may form between cysteine 1430 of DNA-directed RNA polymerase II subunit RPB1 and cysteine 1281 of DNA-directed RNA polymerase subunit beta (1430 and 1155 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
27
PDB code
7oo3
Structure name
pol ii-csb-csa-ddb1-uvssa (structure1)
Structure deposition date
2021-05-26
Thiol separation (Å)
9
Half-sphere exposure sum ?
84
Minimum pKa ?
13
% buried
100
Peptide A name
DNA-directed RNA polymerase II subunit RPB1
Peptide B name
DNA-directed RNA polymerase subunit beta
Peptide A accession
P11414
Peptide B accession
I3LGP4
Peptide A residue number
1430
Peptide B residue number
1281
Ligandability
Cysteine 1430 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 1281 of DNA-directed RNA polymerase subunit beta
5iyb A 1430 B 1155
A redox-regulated disulphide may form between cysteine 1430 of DNA-directed RNA polymerase II subunit RPB1 and cysteine 1155 of DNA-directed RNA polymerase II subunit RPB2. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
26
PDB code
5iyb
Structure name
human core-pic in the open state
Structure deposition date
2016-03-24
Thiol separation (Å)
10
Half-sphere exposure sum ?
80
Minimum pKa ?
12
% buried
100
Peptide A name
DNA-directed RNA polymerase II subunit RPB1
Peptide B name
DNA-directed RNA polymerase II subunit RPB2
Peptide A accession
P24928
Peptide B accession
P30876
Peptide A residue number
1430
Peptide B residue number
1155
Ligandability
Cysteine 1430 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 1155 of DNA-directed RNA polymerase II subunit RPB2
5iy9 A 114 A 184
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 114 and 184.
Details
Redox score ?
80
PDB code
5iy9
Structure name
human holo-pic in the initial transcribing state (no iis)
Structure deposition date
2016-03-24
Thiol separation (Å)
4
Half-sphere exposure sum ?
50
Minimum pKa ?
7
% buried
19
Peptide accession
P24928
Residue number A
114
Residue number B
184
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 114 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 184 of DNA-directed RNA polymerase II subunit RPB1
7b0y A 114 A 154
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 114 and 154.
Details
Redox score ?
78
PDB code
7b0y
Structure name
structure of a transcribing rna polymerase ii-u1 snrnp complex
Structure deposition date
2020-11-23
Thiol separation (Å)
3
Half-sphere exposure sum ?
55
Minimum pKa ?
9
% buried
29
Peptide accession
P11414
Residue number A
114
Residue number B
154
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 114 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 154 of DNA-directed RNA polymerase II subunit RPB1
5iy9 A 111 A 154
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 111 and 154.
Details
Redox score ?
76
PDB code
5iy9
Structure name
human holo-pic in the initial transcribing state (no iis)
Structure deposition date
2016-03-24
Thiol separation (Å)
3
Half-sphere exposure sum ?
72
Minimum pKa ?
11
% buried
40
Peptide accession
P24928
Residue number A
111
Residue number B
154
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 111 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 154 of DNA-directed RNA polymerase II subunit RPB1
5iyb A 111 A 184
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 111 and 184.
Details
Redox score ?
76
PDB code
5iyb
Structure name
human core-pic in the open state
Structure deposition date
2016-03-24
Thiol separation (Å)
4
Half-sphere exposure sum ?
61
Minimum pKa ?
9
% buried
40
Peptide accession
P24928
Residue number A
111
Residue number B
184
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 111 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 184 of DNA-directed RNA polymerase II subunit RPB1
6o9l A 154 A 184
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 154 and 184.
Details
Redox score ?
76
PDB code
6o9l
Structure name
human holo-pic in the closed state
Structure deposition date
2019-03-14
Thiol separation (Å)
6
Half-sphere exposure sum ?
56
Minimum pKa ?
2
% buried
40
Peptide accession
P24928
Residue number A
154
Residue number B
184
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 154 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 184 of DNA-directed RNA polymerase II subunit RPB1
5iyd A 109 A 148
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 109 and 148.
Details
Redox score ?
75
PDB code
5iyd
Structure name
human core-pic in the initial transcribing state (no iis)
Structure deposition date
2016-03-24
Thiol separation (Å)
3
Half-sphere exposure sum ?
70
Minimum pKa ?
8
% buried
86
Peptide accession
P24928
Residue number A
109
Residue number B
148
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 109 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 148 of DNA-directed RNA polymerase II subunit RPB1
7opc A 74 A 81
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 74 and 81.
Details
Redox score ?
74
PDB code
7opc
Structure name
pol ii-csb-crl4csa-uvssa-spt6-paf (structure 4)
Structure deposition date
2021-05-31
Thiol separation (Å)
3
Half-sphere exposure sum ?
72
Minimum pKa ?
10
% buried
80
Peptide accession
P11414
Residue number A
74
Residue number B
81
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 74 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 81 of DNA-directed RNA polymerase II subunit RPB1
5iy7 A 111 A 114
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 111 and 114.
Details
Redox score ?
73
PDB code
5iy7
Structure name
human holo-pic in the open state
Structure deposition date
2016-03-24
Thiol separation (Å)
4
Half-sphere exposure sum ?
70
Minimum pKa ?
8
% buried
36
Peptide accession
P24928
Residue number A
111
Residue number B
114
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 111 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 114 of DNA-directed RNA polymerase II subunit RPB1
7okx A 71 A 74
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 71 and 74. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
59
PDB code
7okx
Structure name
structure of active transcription elongation complex pol ii-dsif (spt5-kow5)-ell2-eaf1 (composite structure)
Structure deposition date
2021-05-18
Thiol separation (Å)
4
Half-sphere exposure sum ?
65
Minimum pKa ?
18
% buried
nan
Peptide accession
P11414
Residue number A
71
Residue number B
74
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 71 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 74 of DNA-directed RNA polymerase II subunit RPB1
5iyd A 71 A 81
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 71 and 81. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
59
PDB code
5iyd
Structure name
human core-pic in the initial transcribing state (no iis)
Structure deposition date
2016-03-24
Thiol separation (Å)
3
Half-sphere exposure sum ?
69
Minimum pKa ?
20
% buried
nan
Peptide accession
P24928
Residue number A
71
Residue number B
81
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 71 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 81 of DNA-directed RNA polymerase II subunit RPB1
5iya A 148 A 154
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 148 and 154. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
40
PDB code
5iya
Structure name
human core-pic in the closed state
Structure deposition date
2016-03-24
Thiol separation (Å)
9
Half-sphere exposure sum ?
67
Minimum pKa ?
11
% buried
60
Peptide accession
P24928
Residue number A
148
Residue number B
154
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 148 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 154 of DNA-directed RNA polymerase II subunit RPB1
6o9l A 602 A 641
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 602 and 641. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
33
PDB code
6o9l
Structure name
human holo-pic in the closed state
Structure deposition date
2019-03-14
Thiol separation (Å)
10
Half-sphere exposure sum ?
77
Minimum pKa ?
9
% buried
93
Peptide accession
P24928
Residue number A
602
Residue number B
641
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 602 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 641 of DNA-directed RNA polymerase II subunit RPB1
5iy9 A 111 A 148
A redox-regulated disulphide may form within DNA-directed RNA polymerase II subunit RPB1 between cysteines 111 and 148. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
30
PDB code
5iy9
Structure name
human holo-pic in the initial transcribing state (no iis)
Structure deposition date
2016-03-24
Thiol separation (Å)
10
Half-sphere exposure sum ?
68
Minimum pKa ?
13
% buried
68
Peptide accession
P24928
Residue number A
111
Residue number B
148
Peptide name
DNA-directed RNA polymerase II subunit RPB1
Ligandability
Cysteine 111 of DNA-directed RNA polymerase II subunit RPB1
Cysteine 148 of DNA-directed RNA polymerase II subunit RPB1
If this tool was useful for finding a disulphide, please cite: