ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

Interleukin-12 subunit beta

Intermolecular
Cysteine 199 and cysteine 96 of Interleukin-12 subunit alpha
Cysteine 197 and cysteine 197
Cysteine 73 of Interleukin-23 subunit alpha and cysteine 199
Cysteine 77 of Interleukin-23 subunit alpha and cysteine 199
Intramolecular
Cysteine 139 and cysteine 331
Cysteine 167 and cysteine 323
Cysteine 170 and cysteine 193
Cysteine 305 and cysteine 331
Cysteine 300 and cysteine 327
Cysteine 288 and cysteine 323
More...
Cysteine 311 and cysteine 328
Cysteine 50 and cysteine 90
Cysteine 131 and cysteine 142
Cysteine 142 and cysteine 193
Cysteine 142 and cysteine 170
Cysteine 131 and cysteine 193
Cysteine 131 and cysteine 170
A redox-regulated disulphide may form between cysteine 199 of Interleukin-12 subunit beta and cysteine 96 of Interleukin-12 subunit alpha (177 and 74 respectively in this structure).

Details

Redox score ?
88
PDB code
1f45
Structure name
human interleukin-12
Structure deposition date
2000-06-07
Thiol separation (Å)
2
Half-sphere exposure sum ?
nan
Minimum pKa ?
nan
% buried
nan
Peptide A name
Interleukin-12 subunit beta
Peptide B name
Interleukin-12 subunit alpha
Peptide A accession
P29460
Peptide B accession
P29459
Peptide A residue number
199
Peptide B residue number
96

Ligandability

Cysteine 199 of Interleukin-12 subunit beta

Cysteine 96 of Interleukin-12 subunit alpha

A redox-regulated disulphide may form between two units of Interleukin-12 subunit beta at cysteines 197 and 197.

Details

Redox score ?
86
PDB code
6smc
Structure name
mouse interleukin-12 subunit beta - p80 homodimer in space group p1
Structure deposition date
2019-08-21
Thiol separation (Å)
2
Half-sphere exposure sum ?
42
Minimum pKa ?
nan
% buried
nan
Peptide A name
Interleukin-12 subunit beta
Peptide B name
Interleukin-12 subunit beta
Peptide A accession
P43432
Peptide B accession
P43432
Peptide A residue number
197
Peptide B residue number
197

Ligandability

A redox-regulated disulphide may form between cysteine 73 of Interleukin-23 subunit alpha and cysteine 199 of Interleukin-12 subunit beta (54 and 177 respectively in this structure).

Details

Redox score ?
82
PDB code
4grw
Structure name
structure of a complex of human il-23 with 3 nanobodies (llama vhhs)
Structure deposition date
2012-08-27
Thiol separation (Å)
3
Half-sphere exposure sum ?
48
Minimum pKa ?
8
% buried
36
Peptide A name
Interleukin-23 subunit alpha
Peptide B name
Interleukin-12 subunit beta
Peptide A accession
Q9NPF7
Peptide B accession
P29460
Peptide A residue number
73
Peptide B residue number
199

Ligandability

Cysteine 73 of Interleukin-23 subunit alpha

Cysteine 199 of Interleukin-12 subunit beta

A redox-regulated disulphide may form between cysteine 77 of Interleukin-23 subunit alpha and cysteine 199 of Interleukin-12 subunit beta (58 and 177 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
38
PDB code
3d85
Structure name
crystal structure of il-23 in complex with neutralizing fab
Structure deposition date
2008-05-22
Thiol separation (Å)
9
Half-sphere exposure sum ?
69
Minimum pKa ?
11
% buried
nan
Peptide A name
Interleukin-23 subunit alpha
Peptide B name
Interleukin-12 subunit beta
Peptide A accession
Q9NPF7
Peptide B accession
P29460
Peptide A residue number
77
Peptide B residue number
199

Ligandability

Cysteine 77 of Interleukin-23 subunit alpha

Cysteine 199 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 139 and 331 (305 and 331 respectively in this structure).

Details

Redox score ?
91
PDB code
7pur
Structure name
mouse interleukin-12 subunit beta - p80 homodimer in space group p21 crystal form 2
Structure deposition date
2021-09-30
Thiol separation (Å)
2
Half-sphere exposure sum ?
nan
Minimum pKa ?
nan
% buried
nan
Peptide accession
P43432
Residue number A
139
Residue number B
331
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 139 of Interleukin-12 subunit beta

Cysteine 331 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 167 and 323 (288 and 323 respectively in this structure).

Details

Redox score ?
89
PDB code
6sff
Structure name
mouse interleukin-12 subunit beta - p80 homodimer in space group i41
Structure deposition date
2019-08-01
Thiol separation (Å)
2
Half-sphere exposure sum ?
nan
Minimum pKa ?
nan
% buried
nan
Peptide accession
P43432
Residue number A
167
Residue number B
323
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 167 of Interleukin-12 subunit beta

Cysteine 323 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 170 and 193 (148 and 171 respectively in this structure).

Details

Redox score ?
88
PDB code
3d87
Structure name
crystal structure of interleukin-23
Structure deposition date
2008-05-22
Thiol separation (Å)
3
Half-sphere exposure sum ?
64
Minimum pKa ?
5
% buried
62
Peptide accession
P29460
Residue number A
170
Residue number B
193
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 170 of Interleukin-12 subunit beta

Cysteine 193 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 305 and 331.

Details

Redox score ?
87
PDB code
6smc
Structure name
mouse interleukin-12 subunit beta - p80 homodimer in space group p1
Structure deposition date
2019-08-21
Thiol separation (Å)
2
Half-sphere exposure sum ?
52
Minimum pKa ?
nan
% buried
nan
Peptide accession
P43432
Residue number A
305
Residue number B
331
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 305 of Interleukin-12 subunit beta

Cysteine 331 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 300 and 327 (278 and 305 respectively in this structure).

Details

Redox score ?
87
PDB code
3d87
Structure name
crystal structure of interleukin-23
Structure deposition date
2008-05-22
Thiol separation (Å)
3
Half-sphere exposure sum ?
54
Minimum pKa ?
8
% buried
6
Peptide accession
P29460
Residue number A
300
Residue number B
327
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 300 of Interleukin-12 subunit beta

Cysteine 327 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 288 and 323.

Details

Redox score ?
86
PDB code
6smc
Structure name
mouse interleukin-12 subunit beta - p80 homodimer in space group p1
Structure deposition date
2019-08-21
Thiol separation (Å)
2
Half-sphere exposure sum ?
56
Minimum pKa ?
nan
% buried
nan
Peptide accession
P43432
Residue number A
288
Residue number B
323
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 288 of Interleukin-12 subunit beta

Cysteine 323 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 311 and 328.

Details

Redox score ?
80
PDB code
6sp3
Structure name
mouse interleukin-12 subunit beta - p80 homodimer in space group p21 crystal form 1
Structure deposition date
2019-08-30
Thiol separation (Å)
2
Half-sphere exposure sum ?
64
Minimum pKa ?
nan
% buried
nan
Peptide accession
P43432
Residue number A
311
Residue number B
328
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 311 of Interleukin-12 subunit beta

Cysteine 328 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 50 and 90 (28 and 68 respectively in this structure).

Details

Redox score ?
79
PDB code
3d87
Structure name
crystal structure of interleukin-23
Structure deposition date
2008-05-22
Thiol separation (Å)
3
Half-sphere exposure sum ?
74
Minimum pKa ?
9
% buried
28
Peptide accession
P29460
Residue number A
50
Residue number B
90
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 50 of Interleukin-12 subunit beta

Cysteine 90 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 131 and 142 (109 and 120 respectively in this structure).

Details

Redox score ?
74
PDB code
3d87
Structure name
crystal structure of interleukin-23
Structure deposition date
2008-05-22
Thiol separation (Å)
3
Half-sphere exposure sum ?
77
Minimum pKa ?
9
% buried
98
Peptide accession
P29460
Residue number A
131
Residue number B
142
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 131 of Interleukin-12 subunit beta

Cysteine 142 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 142 and 193 (120 and 171 respectively in this structure).

Details

Redox score ?
73
PDB code
3d87
Structure name
crystal structure of interleukin-23
Structure deposition date
2008-05-22
Thiol separation (Å)
5
Half-sphere exposure sum ?
78
Minimum pKa ?
5
% buried
84
Peptide accession
P29460
Residue number A
142
Residue number B
193
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 142 of Interleukin-12 subunit beta

Cysteine 193 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 142 and 170 (120 and 148 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
56
PDB code
1f42
Structure name
the p40 domain of human interleukin-12
Structure deposition date
2000-06-07
Thiol separation (Å)
7
Half-sphere exposure sum ?
69
Minimum pKa ?
nan
% buried
nan
Peptide accession
P29460
Residue number A
142
Residue number B
170
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 142 of Interleukin-12 subunit beta

Cysteine 170 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 131 and 193. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
50
PDB code
5njd
Structure name
structure of interleukin 23 in complex with briakinumab fab
Structure deposition date
2017-03-28
Thiol separation (Å)
8
Half-sphere exposure sum ?
68
Minimum pKa ?
nan
% buried
nan
Peptide accession
P29460
Residue number A
131
Residue number B
193
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 131 of Interleukin-12 subunit beta

Cysteine 193 of Interleukin-12 subunit beta

A redox-regulated disulphide may form within Interleukin-12 subunit beta between cysteines 131 and 170. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
43
PDB code
5njd
Structure name
structure of interleukin 23 in complex with briakinumab fab
Structure deposition date
2017-03-28
Thiol separation (Å)
9
Half-sphere exposure sum ?
73
Minimum pKa ?
nan
% buried
nan
Peptide accession
P29460
Residue number A
131
Residue number B
170
Peptide name
Interleukin-12 subunit beta

Ligandability

Cysteine 131 of Interleukin-12 subunit beta

Cysteine 170 of Interleukin-12 subunit beta

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