ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

Excitatory amino acid transporter 2

Intermolecular
Cysteine 184 and cysteine 184
Intramolecular
Cysteine 60 and cysteine 297
Cysteine 294 and cysteine 297
Cysteine 60 and cysteine 294
A redox-regulated disulphide may form between two units of Excitatory amino acid transporter 2 at cysteines 184 and 184. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
27
PDB code
7xr6
Structure name
structure of human excitatory amino acid transporter 2 (eaat2) in complex with way-213613
Structure deposition date
2022-05-09
Thiol separation (Å)
9
Half-sphere exposure sum ?
82
Minimum pKa ?
12
% buried
100
Peptide A name
Excitatory amino acid transporter 2
Peptide B name
Excitatory amino acid transporter 2
Peptide A accession
P43004
Peptide B accession
P43004
Peptide A residue number
184
Peptide B residue number
184

Ligandability

A redox-regulated disulphide may form within Excitatory amino acid transporter 2 between cysteines 60 and 297.

Details

Redox score ?
60
PDB code
7vr7
Structure name
inward-facing structure of human eaat2 in the way213613-bound state
Structure deposition date
2021-10-22
Thiol separation (Å)
5
Half-sphere exposure sum ?
67
Minimum pKa ?
9
% buried
18
Peptide accession
P43004
Residue number A
60
Residue number B
297
Peptide name
Excitatory amino acid transporter 2

Ligandability

Cysteine 60 of Excitatory amino acid transporter 2

Cysteine 297 of Excitatory amino acid transporter 2

A redox-regulated disulphide may form within Excitatory amino acid transporter 2 between cysteines 294 and 297. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
45
PDB code
7vr7
Structure name
inward-facing structure of human eaat2 in the way213613-bound state
Structure deposition date
2021-10-22
Thiol separation (Å)
7
Half-sphere exposure sum ?
81
Minimum pKa ?
11
% buried
44
Peptide accession
P43004
Residue number A
294
Residue number B
297
Peptide name
Excitatory amino acid transporter 2

Ligandability

Cysteine 294 of Excitatory amino acid transporter 2

Cysteine 297 of Excitatory amino acid transporter 2

A redox-regulated disulphide may form within Excitatory amino acid transporter 2 between cysteines 60 and 294. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
38
PDB code
7vr8
Structure name
inward-facing structure of human eaat2 in the substrate-free state
Structure deposition date
2021-10-22
Thiol separation (Å)
9
Half-sphere exposure sum ?
65
Minimum pKa ?
9
% buried
30
Peptide accession
P43004
Residue number A
60
Residue number B
294
Peptide name
Excitatory amino acid transporter 2

Ligandability

Cysteine 60 of Excitatory amino acid transporter 2

Cysteine 294 of Excitatory amino acid transporter 2

If this tool was useful for finding a disulphide, please cite: