ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

DNA replication ATP-dependent helicase/nuclease DNA2

Intramolecular
Cysteine 394 and cysteine 397
Cysteine 137 and cysteine 397
Cysteine 137 and cysteine 394
Cysteine 744 and cysteine 773
Cysteine 394 and cysteine 403 L
Cysteine 397 and cysteine 403 L
Cysteine 137 and cysteine 403 L
Cysteine 81 and cysteine 104
Cysteine 744 and cysteine 760
Cysteine 493 and cysteine 719
Cysteine 760 and cysteine 773
A redox-regulated disulphide may form within DNA replication ATP-dependent helicase/nuclease DNA2 between cysteines 394 and 397.

Details

Redox score ?
61
PDB code
5eaw
Structure name
crystal structure of dna2 nuclease-helicase
Structure deposition date
2015-10-17
Thiol separation (Å)
7
Half-sphere exposure sum ?
51
Minimum pKa ?
10
% buried
44
Peptide accession
Q6ZQJ5
Residue number A
394
Residue number B
397
Peptide name
DNA replication ATP-dependent helicase/nuclease DNA2

Ligandability

Cysteine 394 of DNA replication ATP-dependent helicase/nuclease DNA2

Cysteine 397 of DNA replication ATP-dependent helicase/nuclease DNA2

A redox-regulated disulphide may form within DNA replication ATP-dependent helicase/nuclease DNA2 between cysteines 137 and 397. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
60
PDB code
5ean
Structure name
crystal structure of dna2 in complex with a 5' overhang dna
Structure deposition date
2015-10-16
Thiol separation (Å)
6
Half-sphere exposure sum ?
59
Minimum pKa ?
10
% buried
75
Peptide accession
Q6ZQJ5
Residue number A
137
Residue number B
397
Peptide name
DNA replication ATP-dependent helicase/nuclease DNA2

Ligandability

Cysteine 137 of DNA replication ATP-dependent helicase/nuclease DNA2

Cysteine 397 of DNA replication ATP-dependent helicase/nuclease DNA2

A redox-regulated disulphide may form within DNA replication ATP-dependent helicase/nuclease DNA2 between cysteines 137 and 394. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
58
PDB code
5eax
Structure name
crystal structure of dna2 in complex with an ssdna
Structure deposition date
2015-10-17
Thiol separation (Å)
6
Half-sphere exposure sum ?
57
Minimum pKa ?
11
% buried
58
Peptide accession
Q6ZQJ5
Residue number A
137
Residue number B
394
Peptide name
DNA replication ATP-dependent helicase/nuclease DNA2

Ligandability

Cysteine 137 of DNA replication ATP-dependent helicase/nuclease DNA2

Cysteine 394 of DNA replication ATP-dependent helicase/nuclease DNA2

A redox-regulated disulphide may form within DNA replication ATP-dependent helicase/nuclease DNA2 between cysteines 744 and 773. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
55
PDB code
5eaw
Structure name
crystal structure of dna2 nuclease-helicase
Structure deposition date
2015-10-17
Thiol separation (Å)
5
Half-sphere exposure sum ?
84
Minimum pKa ?
12
% buried
100
Peptide accession
Q6ZQJ5
Residue number A
744
Residue number B
773
Peptide name
DNA replication ATP-dependent helicase/nuclease DNA2

Ligandability

Cysteine 744 of DNA replication ATP-dependent helicase/nuclease DNA2

Cysteine 773 of DNA replication ATP-dependent helicase/nuclease DNA2

A redox-regulated disulphide may form within DNA replication ATP-dependent helicase/nuclease DNA2 between cysteines 394 and 403. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
54
PDB code
5eaw
Structure name
crystal structure of dna2 nuclease-helicase
Structure deposition date
2015-10-17
Thiol separation (Å)
7
Half-sphere exposure sum ?
65
Minimum pKa ?
10
% buried
58
Peptide accession
Q6ZQJ5
Residue number A
394
Residue number B
403
Peptide name
DNA replication ATP-dependent helicase/nuclease DNA2

Ligandability

Cysteine 394 of DNA replication ATP-dependent helicase/nuclease DNA2

Cysteine 403 of DNA replication ATP-dependent helicase/nuclease DNA2

A redox-regulated disulphide may form within DNA replication ATP-dependent helicase/nuclease DNA2 between cysteines 397 and 403. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
54
PDB code
5ean
Structure name
crystal structure of dna2 in complex with a 5' overhang dna
Structure deposition date
2015-10-16
Thiol separation (Å)
7
Half-sphere exposure sum ?
67
Minimum pKa ?
11
% buried
80
Peptide accession
Q6ZQJ5
Residue number A
397
Residue number B
403
Peptide name
DNA replication ATP-dependent helicase/nuclease DNA2

Ligandability

Cysteine 397 of DNA replication ATP-dependent helicase/nuclease DNA2

Cysteine 403 of DNA replication ATP-dependent helicase/nuclease DNA2

A redox-regulated disulphide may form within DNA replication ATP-dependent helicase/nuclease DNA2 between cysteines 137 and 403. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
51
PDB code
5eax
Structure name
crystal structure of dna2 in complex with an ssdna
Structure deposition date
2015-10-17
Thiol separation (Å)
7
Half-sphere exposure sum ?
73
Minimum pKa ?
10
% buried
78
Peptide accession
Q6ZQJ5
Residue number A
137
Residue number B
403
Peptide name
DNA replication ATP-dependent helicase/nuclease DNA2

Ligandability

Cysteine 137 of DNA replication ATP-dependent helicase/nuclease DNA2

Cysteine 403 of DNA replication ATP-dependent helicase/nuclease DNA2

A redox-regulated disulphide may form within DNA replication ATP-dependent helicase/nuclease DNA2 between cysteines 81 and 104. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
46
PDB code
5eax
Structure name
crystal structure of dna2 in complex with an ssdna
Structure deposition date
2015-10-17
Thiol separation (Å)
8
Half-sphere exposure sum ?
61
Minimum pKa ?
10
% buried
60
Peptide accession
Q6ZQJ5
Residue number A
81
Residue number B
104
Peptide name
DNA replication ATP-dependent helicase/nuclease DNA2

Ligandability

Cysteine 81 of DNA replication ATP-dependent helicase/nuclease DNA2

Cysteine 104 of DNA replication ATP-dependent helicase/nuclease DNA2

A redox-regulated disulphide may form within DNA replication ATP-dependent helicase/nuclease DNA2 between cysteines 744 and 760. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
42
PDB code
5eaw
Structure name
crystal structure of dna2 nuclease-helicase
Structure deposition date
2015-10-17
Thiol separation (Å)
7
Half-sphere exposure sum ?
84
Minimum pKa ?
12
% buried
100
Peptide accession
Q6ZQJ5
Residue number A
744
Residue number B
760
Peptide name
DNA replication ATP-dependent helicase/nuclease DNA2

Ligandability

Cysteine 744 of DNA replication ATP-dependent helicase/nuclease DNA2

Cysteine 760 of DNA replication ATP-dependent helicase/nuclease DNA2

A redox-regulated disulphide may form within DNA replication ATP-dependent helicase/nuclease DNA2 between cysteines 493 and 719. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
39
PDB code
5eaw
Structure name
crystal structure of dna2 nuclease-helicase
Structure deposition date
2015-10-17
Thiol separation (Å)
10
Half-sphere exposure sum ?
54
Minimum pKa ?
10
% buried
59
Peptide accession
Q6ZQJ5
Residue number A
493
Residue number B
719
Peptide name
DNA replication ATP-dependent helicase/nuclease DNA2

Ligandability

Cysteine 493 of DNA replication ATP-dependent helicase/nuclease DNA2

Cysteine 719 of DNA replication ATP-dependent helicase/nuclease DNA2

A redox-regulated disulphide may form within DNA replication ATP-dependent helicase/nuclease DNA2 between cysteines 760 and 773. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
36
PDB code
5eaw
Structure name
crystal structure of dna2 nuclease-helicase
Structure deposition date
2015-10-17
Thiol separation (Å)
8
Half-sphere exposure sum ?
87
Minimum pKa ?
13
% buried
100
Peptide accession
Q6ZQJ5
Residue number A
760
Residue number B
773
Peptide name
DNA replication ATP-dependent helicase/nuclease DNA2

Ligandability

Cysteine 760 of DNA replication ATP-dependent helicase/nuclease DNA2

Cysteine 773 of DNA replication ATP-dependent helicase/nuclease DNA2

If this tool was useful for finding a disulphide, please cite: