ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

Ubiquitin-conjugating enzyme E2 D3

Intramolecular
Cysteine 51 and cysteine 85
Cysteine 21 and cysteine 107
Cysteine 85 and cysteine 47
Cysteine 85 and cysteine 75
Cysteine 107 and cysteine 111
Cysteine 21 and cysteine 111
Cysteine 85 and cysteine 72
A redox-regulated disulphide may form within Ubiquitin-conjugating enzyme E2 D3 between cysteines 51 and 85 (51 and 54 respectively in this structure).

Details

Redox score ?
81
PDB code
4r8p
Structure name
crystal structure of the ring1b/bmi1/ubch5c prc1 ubiquitylation module bound to the nucleosome core particle
Structure deposition date
2014-09-02
Thiol separation (Å)
4
Half-sphere exposure sum ?
75
Minimum pKa ?
5
% buried
nan
Peptide accession
P61077
Residue number A
51
Residue number B
85
Peptide name
Ubiquitin-conjugating enzyme E2 D3

Ligandability

Cysteine 51 of Ubiquitin-conjugating enzyme E2 D3

Cysteine 85 of Ubiquitin-conjugating enzyme E2 D3

Cysteine 51 in protein A could not be asigned to a Uniprot residue.
A redox-regulated disulphide may form within Ubiquitin-conjugating enzyme E2 D3 between cysteines 21 and 107.

Details

Redox score ?
80
PDB code
1x23
Structure name
crystal structure of ubch5c
Structure deposition date
2005-04-19
Thiol separation (Å)
3
Half-sphere exposure sum ?
71
Minimum pKa ?
8
% buried
75
Peptide accession
P61077
Residue number A
21
Residue number B
107
Peptide name
Ubiquitin-conjugating enzyme E2 D3

Ligandability

Cysteine 21 of Ubiquitin-conjugating enzyme E2 D3

Cysteine 107 of Ubiquitin-conjugating enzyme E2 D3

A redox-regulated disulphide may form within Ubiquitin-conjugating enzyme E2 D3 between cysteines 85 and 47 (27 and 47 respectively in this structure).

Details

Redox score ?
75
PDB code
7jzv
Structure name
cryo-em structure of the brca1-ubch5c/bard1 e3-e2 module bound to a nucleosome
Structure deposition date
2020-09-02
Thiol separation (Å)
4
Half-sphere exposure sum ?
71
Minimum pKa ?
7
% buried
66
Peptide accession
P61077
Residue number A
85
Residue number B
47
Peptide name
Ubiquitin-conjugating enzyme E2 D3

Ligandability

Cysteine 85 of Ubiquitin-conjugating enzyme E2 D3

Cysteine 47 of Ubiquitin-conjugating enzyme E2 D3

Cysteine 47 in protein B could not be asigned to a Uniprot residue.
A redox-regulated disulphide may form within Ubiquitin-conjugating enzyme E2 D3 between cysteines 85 and 75 (54 and 75 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
60
PDB code
4r8p
Structure name
crystal structure of the ring1b/bmi1/ubch5c prc1 ubiquitylation module bound to the nucleosome core particle
Structure deposition date
2014-09-02
Thiol separation (Å)
4
Half-sphere exposure sum ?
82
Minimum pKa ?
14
% buried
68
Peptide accession
P61077
Residue number A
85
Residue number B
75
Peptide name
Ubiquitin-conjugating enzyme E2 D3

Ligandability

Cysteine 85 of Ubiquitin-conjugating enzyme E2 D3

Cysteine 75 of Ubiquitin-conjugating enzyme E2 D3

Cysteine 75 in protein B could not be asigned to a Uniprot residue.
A redox-regulated disulphide may form within Ubiquitin-conjugating enzyme E2 D3 between cysteines 107 and 111. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
53
PDB code
6cp0
Structure name
sdca in complex with the e2, ubch5c
Structure deposition date
2018-03-13
Thiol separation (Å)
9
Half-sphere exposure sum ?
57
Minimum pKa ?
7
% buried
26
Peptide accession
P61077
Residue number A
107
Residue number B
111
Peptide name
Ubiquitin-conjugating enzyme E2 D3

Ligandability

Cysteine 107 of Ubiquitin-conjugating enzyme E2 D3

Cysteine 111 of Ubiquitin-conjugating enzyme E2 D3

A redox-regulated disulphide may form within Ubiquitin-conjugating enzyme E2 D3 between cysteines 21 and 111. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
35
PDB code
1x23
Structure name
crystal structure of ubch5c
Structure deposition date
2005-04-19
Thiol separation (Å)
10
Half-sphere exposure sum ?
61
Minimum pKa ?
12
% buried
50
Peptide accession
P61077
Residue number A
21
Residue number B
111
Peptide name
Ubiquitin-conjugating enzyme E2 D3

Ligandability

Cysteine 21 of Ubiquitin-conjugating enzyme E2 D3

Cysteine 111 of Ubiquitin-conjugating enzyme E2 D3

A redox-regulated disulphide may form within Ubiquitin-conjugating enzyme E2 D3 between cysteines 85 and 72 (54 and 72 respectively in this structure).

Details

Redox score ?
nan
PDB code
4r8p
Structure name
crystal structure of the ring1b/bmi1/ubch5c prc1 ubiquitylation module bound to the nucleosome core particle
Structure deposition date
2014-09-02
Thiol separation (Å)
4
Half-sphere exposure sum ?
79
Minimum pKa ?
5
% buried
72
Peptide accession
P61077
Residue number A
85
Residue number B
72
Peptide name
Ubiquitin-conjugating enzyme E2 D3

Ligandability

Cysteine 85 of Ubiquitin-conjugating enzyme E2 D3

Cysteine 72 of Ubiquitin-conjugating enzyme E2 D3

Cysteine 72 in protein B could not be asigned to a Uniprot residue.
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