ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

Tyrosine-protein kinase ITK/TSK

Intramolecular
Cysteine 133 and cysteine 143
Cysteine 132 and cysteine 133
Cysteine 132 and cysteine 143
Cysteine 425 and cysteine 432
Cysteine 442 and cysteine 488
Cysteine 467 and cysteine 594
Cysteine 463 and cysteine 467
Cysteine 463 and cysteine 594
Cysteine 463 and cysteine 488
A redox-regulated disulphide may form within Tyrosine-protein kinase ITK/TSK between cysteines 133 and 143 (28 and 38 respectively in this structure).

Details

Redox score ?
86
PDB code
2e6i
Structure name
solution structure of the btk motif of tyrosine-protein kinase itk from human
Structure deposition date
2006-12-27
Thiol separation (Å)
4
Half-sphere exposure sum ?
45
Minimum pKa ?
6
% buried
0
Peptide accession
Q08881
Residue number A
133
Residue number B
143
Peptide name
Tyrosine-protein kinase ITK/TSK

Ligandability

Cysteine 133 of Tyrosine-protein kinase ITK/TSK

Cysteine 143 of Tyrosine-protein kinase ITK/TSK

A redox-regulated disulphide may form within Tyrosine-protein kinase ITK/TSK between cysteines 132 and 133 (27 and 28 respectively in this structure).

Details

Redox score ?
85
PDB code
2e6i
Structure name
solution structure of the btk motif of tyrosine-protein kinase itk from human
Structure deposition date
2006-12-27
Thiol separation (Å)
4
Half-sphere exposure sum ?
42
Minimum pKa ?
6
% buried
0
Peptide accession
Q08881
Residue number A
132
Residue number B
133
Peptide name
Tyrosine-protein kinase ITK/TSK

Ligandability

Cysteine 132 of Tyrosine-protein kinase ITK/TSK

Cysteine 133 of Tyrosine-protein kinase ITK/TSK

A redox-regulated disulphide may form within Tyrosine-protein kinase ITK/TSK between cysteines 132 and 143 (27 and 38 respectively in this structure).

Details

Redox score ?
83
PDB code
2e6i
Structure name
solution structure of the btk motif of tyrosine-protein kinase itk from human
Structure deposition date
2006-12-27
Thiol separation (Å)
4
Half-sphere exposure sum ?
43
Minimum pKa ?
8
% buried
0
Peptide accession
Q08881
Residue number A
132
Residue number B
143
Peptide name
Tyrosine-protein kinase ITK/TSK

Ligandability

Cysteine 132 of Tyrosine-protein kinase ITK/TSK

Cysteine 143 of Tyrosine-protein kinase ITK/TSK

A redox-regulated disulphide may form within Tyrosine-protein kinase ITK/TSK between cysteines 425 and 432.

Details

Redox score ?
77
PDB code
3qgy
Structure name
crystal structure of itk inhibitor complex
Structure deposition date
2011-01-25
Thiol separation (Å)
3
Half-sphere exposure sum ?
72
Minimum pKa ?
8
% buried
57
Peptide accession
Q08881
Residue number A
425
Residue number B
432
Peptide name
Tyrosine-protein kinase ITK/TSK

Ligandability

Cysteine 425 of Tyrosine-protein kinase ITK/TSK

Cysteine 432 of Tyrosine-protein kinase ITK/TSK

A redox-regulated disulphide may form within Tyrosine-protein kinase ITK/TSK between cysteines 442 and 488. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
36
PDB code
3qgw
Structure name
crystal structure of itk kinase bound to an inhibitor
Structure deposition date
2011-01-25
Thiol separation (Å)
9
Half-sphere exposure sum ?
75
Minimum pKa ?
11
% buried
85
Peptide accession
Q08881
Residue number A
442
Residue number B
488
Peptide name
Tyrosine-protein kinase ITK/TSK

Ligandability

Cysteine 442 of Tyrosine-protein kinase ITK/TSK

Cysteine 488 of Tyrosine-protein kinase ITK/TSK

A redox-regulated disulphide may form within Tyrosine-protein kinase ITK/TSK between cysteines 467 and 594. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
36
PDB code
1snx
Structure name
crystal structure of apo interleukin-2 tyrosine kinase catalytic domain
Structure deposition date
2004-03-12
Thiol separation (Å)
8
Half-sphere exposure sum ?
82
Minimum pKa ?
12
% buried
99
Peptide accession
Q08881
Residue number A
467
Residue number B
594
Peptide name
Tyrosine-protein kinase ITK/TSK

Ligandability

Cysteine 467 of Tyrosine-protein kinase ITK/TSK

Cysteine 594 of Tyrosine-protein kinase ITK/TSK

A redox-regulated disulphide may form within Tyrosine-protein kinase ITK/TSK between cysteines 463 and 467. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
30
PDB code
3v5l
Structure name
crystal structure of interleukin-2 inducible t-cell kinase itk catalytic domain with thienopyrazolylindole inhibitor 542
Structure deposition date
2011-12-16
Thiol separation (Å)
9
Half-sphere exposure sum ?
90
Minimum pKa ?
13
% buried
96
Peptide accession
Q08881
Residue number A
463
Residue number B
467
Peptide name
Tyrosine-protein kinase ITK/TSK

Ligandability

Cysteine 463 of Tyrosine-protein kinase ITK/TSK

Cysteine 467 of Tyrosine-protein kinase ITK/TSK

A redox-regulated disulphide may form within Tyrosine-protein kinase ITK/TSK between cysteines 463 and 594. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
26
PDB code
4ppc
Structure name
itk kinase domain with compound 27 (n-{1-[(1r)-3-(dimethylamino)-1- phenylpropyl]-1h-pyrazol-4-yl}-6-(1h-pyrazol-4-yl)-1h-indazole-3- carboxamide)
Structure deposition date
2014-02-26
Thiol separation (Å)
10
Half-sphere exposure sum ?
82
Minimum pKa ?
13
% buried
100
Peptide accession
Q08881
Residue number A
463
Residue number B
594
Peptide name
Tyrosine-protein kinase ITK/TSK

Ligandability

Cysteine 463 of Tyrosine-protein kinase ITK/TSK

Cysteine 594 of Tyrosine-protein kinase ITK/TSK

A redox-regulated disulphide may form within Tyrosine-protein kinase ITK/TSK between cysteines 463 and 488. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
25
PDB code
4l7s
Structure name
kinase domain mutant of human itk in complex with an aminobenzothiazole inhibitor
Structure deposition date
2013-06-14
Thiol separation (Å)
10
Half-sphere exposure sum ?
87
Minimum pKa ?
13
% buried
100
Peptide accession
Q08881
Residue number A
463
Residue number B
488
Peptide name
Tyrosine-protein kinase ITK/TSK

Ligandability

Cysteine 463 of Tyrosine-protein kinase ITK/TSK

Cysteine 488 of Tyrosine-protein kinase ITK/TSK

If this tool was useful for finding a disulphide, please cite: