ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

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Pre-mRNA-processing-splicing factor 8

Intermolecular
Cysteine 719 and cysteine 250 of SNW domain-containing protein 1 L
Intramolecular
Cysteine 2216 and cysteine 2223 L
Cysteine 956 and cysteine 1194 L
Cysteine 956 and cysteine 1228 L
Cysteine 1194 and cysteine 1228 L
Cysteine 2116 and cysteine 2223 L
Cysteine 1089 and cysteine 1190
Cysteine 1133 and cysteine 1194
Cysteine 2116 and cysteine 2216 L
Cysteine 1190 and cysteine 1194
A redox-regulated disulphide may form between cysteine 719 of Pre-mRNA-processing-splicing factor 8 and cysteine 250 of SNW domain-containing protein 1. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
35
PDB code
6icz
Structure name
cryo-em structure of a human post-catalytic spliceosome (p complex) at 3
Structure deposition date
2018-09-07
Thiol separation (Å)
9
Half-sphere exposure sum ?
64
Minimum pKa ?
13
% buried
96
Peptide A name
Pre-mRNA-processing-splicing factor 8
Peptide B name
SNW domain-containing protein 1
Peptide A accession
Q6P2Q9
Peptide B accession
Q13573
Peptide A residue number
719
Peptide B residue number
250

Ligandability

Cysteine 719 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 250 of SNW domain-containing protein 1

Ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Ligandable in the dataset of Backus et al.

Ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Pre-mRNA-processing-splicing factor 8 between cysteines 2216 and 2223.

Details

Redox score ?
77
PDB code
7dvq
Structure name
cryo-em structure of the activated human minor spliceosome (minor bact complex)
Structure deposition date
2021-01-14
Thiol separation (Å)
3
Half-sphere exposure sum ?
76
Minimum pKa ?
8
% buried
90
Peptide accession
Q6P2Q9
Residue number A
2216
Residue number B
2223
Peptide name
Pre-mRNA-processing-splicing factor 8

Ligandability

Cysteine 2216 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

Cysteine 2223 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Pre-mRNA-processing-splicing factor 8 between cysteines 956 and 1194.

Details

Redox score ?
76
PDB code
6id0
Structure name
cryo-em structure of a human intron lariat spliceosome prior to prp43 loaded (ils1 complex) at 2
Structure deposition date
2018-09-07
Thiol separation (Å)
4
Half-sphere exposure sum ?
78
Minimum pKa ?
5
% buried
100
Peptide accession
Q6P2Q9
Residue number A
956
Residue number B
1194
Peptide name
Pre-mRNA-processing-splicing factor 8

Ligandability

Cysteine 956 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

Cysteine 1194 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Pre-mRNA-processing-splicing factor 8 between cysteines 956 and 1228.

Details

Redox score ?
71
PDB code
7dvq
Structure name
cryo-em structure of the activated human minor spliceosome (minor bact complex)
Structure deposition date
2021-01-14
Thiol separation (Å)
3
Half-sphere exposure sum ?
77
Minimum pKa ?
9
% buried
100
Peptide accession
Q6P2Q9
Residue number A
956
Residue number B
1228
Peptide name
Pre-mRNA-processing-splicing factor 8

Ligandability

Cysteine 956 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

Cysteine 1228 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Pre-mRNA-processing-splicing factor 8 between cysteines 1194 and 1228. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
55
PDB code
6id1
Structure name
cryo-em structure of a human intron lariat spliceosome after prp43 loaded (ils2 complex) at 2
Structure deposition date
2018-09-07
Thiol separation (Å)
4
Half-sphere exposure sum ?
75
Minimum pKa ?
15
% buried
100
Peptide accession
Q6P2Q9
Residue number A
1194
Residue number B
1228
Peptide name
Pre-mRNA-processing-splicing factor 8

Ligandability

Cysteine 1194 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 1228 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Pre-mRNA-processing-splicing factor 8 between cysteines 2116 and 2223. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
44
PDB code
7bdi
Structure name
human brr2 helicase region in complex with c-tail deleted jab1 and atpgammas
Structure deposition date
2020-12-21
Thiol separation (Å)
9
Half-sphere exposure sum ?
71
Minimum pKa ?
8
% buried
76
Peptide accession
Q6P2Q9
Residue number A
2116
Residue number B
2223
Peptide name
Pre-mRNA-processing-splicing factor 8

Ligandability

Cysteine 2116 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

Cysteine 2223 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Pre-mRNA-processing-splicing factor 8 between cysteines 1089 and 1190. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
43
PDB code
7w5a
Structure name
the cryo-em structure of human pre-c*-ii complex
Structure deposition date
2021-11-29
Thiol separation (Å)
7
Half-sphere exposure sum ?
78
Minimum pKa ?
12
% buried
100
Peptide accession
Q6P2Q9
Residue number A
1089
Residue number B
1190
Peptide name
Pre-mRNA-processing-splicing factor 8

Ligandability

Cysteine 1089 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 1190 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Pre-mRNA-processing-splicing factor 8 between cysteines 1133 and 1194. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
31
PDB code
7dvq
Structure name
cryo-em structure of the activated human minor spliceosome (minor bact complex)
Structure deposition date
2021-01-14
Thiol separation (Å)
10
Half-sphere exposure sum ?
71
Minimum pKa ?
10
% buried
86
Peptide accession
Q6P2Q9
Residue number A
1133
Residue number B
1194
Peptide name
Pre-mRNA-processing-splicing factor 8

Ligandability

Cysteine 1133 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 1194 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Pre-mRNA-processing-splicing factor 8 between cysteines 2116 and 2216. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
26
PDB code
7bdl
Structure name
human brr2 helicase region in complex with c-tail deleted jab1 and mant-adp
Structure deposition date
2020-12-21
Thiol separation (Å)
10
Half-sphere exposure sum ?
78
Minimum pKa ?
12
% buried
96
Peptide accession
Q6P2Q9
Residue number A
2116
Residue number B
2216
Peptide name
Pre-mRNA-processing-splicing factor 8

Ligandability

Cysteine 2116 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

Cysteine 2216 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Pre-mRNA-processing-splicing factor 8 between cysteines 1190 and 1194. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
25
PDB code
7w59
Structure name
the cryo-em structure of human pre-c*-i complex
Structure deposition date
2021-11-29
Thiol separation (Å)
10
Half-sphere exposure sum ?
74
Minimum pKa ?
13
% buried
100
Peptide accession
Q6P2Q9
Residue number A
1190
Residue number B
1194
Peptide name
Pre-mRNA-processing-splicing factor 8

Ligandability

Cysteine 1190 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 1194 of Pre-mRNA-processing-splicing factor 8

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

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