Transcription factor SPT20 homolog

Intermolecular

Cysteine 237 and cysteine 188 of TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L L

Intramolecular

Cysteine 341 and cysteine 419

Cysteine 138 and cysteine 140 L

A redox-regulated disulphide may form between cysteine 237 of Transcription factor SPT20 homolog and cysteine 188 of TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?

PDB code

8h7g

Structure name

cryo-em structure of the human saga complex

Structure deposition date

2022-10-20

Thiol separation (Å)

Half-sphere exposure sum ?

Minimum pK_a ?

% buried

Peptide A name

Transcription factor SPT20 homolog

Peptide B name

TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L

Peptide A accession

Q8NEM7

Peptide B accession

O75529

Peptide A residue number

237

Peptide B residue number

188

Ligandability

Cysteine 237 of Transcription factor SPT20 homolog

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 188 of TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Transcription factor SPT20 homolog between cysteines 341 and 419.

Details

Redox score ?

PDB code

7ktr

Structure name

cryo-em structure of the human saga coactivator complex (trrap, core)

Structure deposition date

2020-11-24

Thiol separation (Å)

Half-sphere exposure sum ?

Minimum pK_a ?

% buried

Peptide accession

Q8NEM7

Residue number A

341

Residue number B

419

Peptide name

Transcription factor SPT20 homolog

Ligandability

Cysteine 341 of Transcription factor SPT20 homolog

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 419 of Transcription factor SPT20 homolog

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Transcription factor SPT20 homolog between cysteines 138 and 140. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?