WD repeat-containing protein 48
Intermolecular
Cysteine 200 and cysteine 48 of Ubiquitin carboxyl-terminal hydrolase 12
Cysteine 200 and cysteine 200
Cysteine 366 and cysteine 233 of Ubiquitin carboxyl-terminal hydrolase 46
Intramolecular
Cysteine 84 and cysteine 85
Cysteine 291 and cysteine 350
Cysteine 225 and cysteine 244
Cysteine 278 and cysteine 291
5k1a D 200 E 52
A redox-regulated disulphide may form between cysteine 200 of WD repeat-containing protein 48 and cysteine 48 of Ubiquitin carboxyl-terminal hydrolase 12 (200 and 52 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
52
PDB code
5k1a
Structure name
crystal structure of the uaf1-usp12 complex in c2 space group
Structure deposition date
2016-05-18
Thiol separation (Å)
7
Half-sphere exposure sum ?
67
Minimum pKa ?
10
% buried
72
Peptide A name
WD repeat-containing protein 48
Peptide B name
Ubiquitin carboxyl-terminal hydrolase 12
Peptide A accession
Q8TAF3
Peptide B accession
O75317
Peptide A residue number
200
Peptide B residue number
48
Ligandability
Cysteine 200 of WD repeat-containing protein 48
Cysteine 48 of Ubiquitin carboxyl-terminal hydrolase 12
7ay2 A 200 D 200
A redox-regulated disulphide may form between two units of WD repeat-containing protein 48 at cysteines 200 and 200. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
42
PDB code
7ay2
Structure name
crystal structure of truncated usp1-uaf1 reacted with ubiquitin-prg
Structure deposition date
2020-11-10
Thiol separation (Å)
9
Half-sphere exposure sum ?
65
Minimum pKa ?
10
% buried
73
Peptide A name
WD repeat-containing protein 48
Peptide B name
WD repeat-containing protein 48
Peptide A accession
Q8TAF3
Peptide B accession
Q8TAF3
Peptide A residue number
200
Peptide B residue number
200
Ligandability
5cvn A 366 B 233
A redox-regulated disulphide may form between cysteine 366 of WD repeat-containing protein 48 and cysteine 233 of Ubiquitin carboxyl-terminal hydrolase 46. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
34
PDB code
5cvn
Structure name
wdr48 (2-580):usp46~ubiquitin ternary complex
Structure deposition date
2015-07-27
Thiol separation (Å)
10
Half-sphere exposure sum ?
66
Minimum pKa ?
11
% buried
90
Peptide A name
WD repeat-containing protein 48
Peptide B name
Ubiquitin carboxyl-terminal hydrolase 46
Peptide A accession
Q8TAF3
Peptide B accession
P62068
Peptide A residue number
366
Peptide B residue number
233
Ligandability
Cysteine 366 of WD repeat-containing protein 48
Cysteine 233 of Ubiquitin carboxyl-terminal hydrolase 46
5l8e A 84 A 85
A redox-regulated disulphide may form within WD repeat-containing protein 48 between cysteines 84 and 85.
Details
Redox score ?
75
PDB code
5l8e
Structure name
structure of uaf1
Structure deposition date
2016-06-07
Thiol separation (Å)
4
Half-sphere exposure sum ?
65
Minimum pKa ?
7
% buried
60
Peptide accession
Q8TAF3
Residue number A
84
Residue number B
85
Peptide name
WD repeat-containing protein 48
Ligandability
Cysteine 84 of WD repeat-containing protein 48
Cysteine 85 of WD repeat-containing protein 48
5k1a H 291 H 350
A redox-regulated disulphide may form within WD repeat-containing protein 48 between cysteines 291 and 350. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
58
PDB code
5k1a
Structure name
crystal structure of the uaf1-usp12 complex in c2 space group
Structure deposition date
2016-05-18
Thiol separation (Å)
6
Half-sphere exposure sum ?
60
Minimum pKa ?
10
% buried
58
Peptide accession
Q8TAF3
Residue number A
291
Residue number B
350
Peptide name
WD repeat-containing protein 48
Ligandability
Cysteine 291 of WD repeat-containing protein 48
Cysteine 350 of WD repeat-containing protein 48
5k1a B 225 B 244
A redox-regulated disulphide may form within WD repeat-containing protein 48 between cysteines 225 and 244. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
39
PDB code
5k1a
Structure name
crystal structure of the uaf1-usp12 complex in c2 space group
Structure deposition date
2016-05-18
Thiol separation (Å)
10
Half-sphere exposure sum ?
71
Minimum pKa ?
8
% buried
70
Peptide accession
Q8TAF3
Residue number A
225
Residue number B
244
Peptide name
WD repeat-containing protein 48
Ligandability
Cysteine 225 of WD repeat-containing protein 48
Cysteine 244 of WD repeat-containing protein 48
5k1b B 278 B 291
A redox-regulated disulphide may form within WD repeat-containing protein 48 between cysteines 278 and 291. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?
Details
Redox score ?
33
PDB code
5k1b
Structure name
crystal structure of the uaf1/usp12 complex in f222 space group
Structure deposition date
2016-05-18
Thiol separation (Å)
10
Half-sphere exposure sum ?
68
Minimum pKa ?
11
% buried
60
Peptide accession
Q8TAF3
Residue number A
278
Residue number B
291
Peptide name
WD repeat-containing protein 48
Ligandability
Cysteine 278 of WD repeat-containing protein 48
Cysteine 291 of WD repeat-containing protein 48
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