ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

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DNA-directed RNA polymerase I subunit RPA49

Intermolecular
Cysteine 535 of DNA-directed RNA polymerase I subunit RPA2 and cysteine 85
Cysteine 535 of DNA-directed RNA polymerase I subunit RPA2 and cysteine 81
Intramolecular
Cysteine 81 and cysteine 85
A redox-regulated disulphide may form between cysteine 535 of DNA-directed RNA polymerase I subunit RPA2 and cysteine 85 of DNA-directed RNA polymerase I subunit RPA49.

Details

Redox score ?
71
PDB code
7ob9
Structure name
cryo-em structure of human rna polymerase i in elongation state
Structure deposition date
2021-04-21
Thiol separation (Å)
5
Half-sphere exposure sum ?
77
Minimum pKa ?
7
% buried
88
Peptide A name
DNA-directed RNA polymerase I subunit RPA2
Peptide B name
DNA-directed RNA polymerase I subunit RPA49
Peptide A accession
Q9H9Y6
Peptide B accession
Q9GZS1
Peptide A residue number
535
Peptide B residue number
85

Ligandability

Cysteine 535 of DNA-directed RNA polymerase I subunit RPA2

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 85 of DNA-directed RNA polymerase I subunit RPA49

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form between cysteine 535 of DNA-directed RNA polymerase I subunit RPA2 and cysteine 81 of DNA-directed RNA polymerase I subunit RPA49. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
41
PDB code
8a43
Structure name
human rna polymerase i
Structure deposition date
2022-06-10
Thiol separation (Å)
8
Half-sphere exposure sum ?
76
Minimum pKa ?
11
% buried
85
Peptide A name
DNA-directed RNA polymerase I subunit RPA2
Peptide B name
DNA-directed RNA polymerase I subunit RPA49
Peptide A accession
Q9H9Y6
Peptide B accession
Q9GZS1
Peptide A residue number
535
Peptide B residue number
81

Ligandability

Cysteine 535 of DNA-directed RNA polymerase I subunit RPA2

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 81 of DNA-directed RNA polymerase I subunit RPA49

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within DNA-directed RNA polymerase I subunit RPA49 between cysteines 81 and 85. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
56
PDB code
7ob9
Structure name
cryo-em structure of human rna polymerase i in elongation state
Structure deposition date
2021-04-21
Thiol separation (Å)
7
Half-sphere exposure sum ?
74
Minimum pKa ?
7
% buried
94
Peptide accession
Q9GZS1
Residue number A
81
Residue number B
85
Peptide name
DNA-directed RNA polymerase I subunit RPA49

Ligandability

Cysteine 81 of DNA-directed RNA polymerase I subunit RPA49

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 85 of DNA-directed RNA polymerase I subunit RPA49

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

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