ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

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Mediator of RNA polymerase II transcription subunit 20

Intramolecular
Cysteine 75 and cysteine 84
Cysteine 147 and cysteine 153
Cysteine 5 and cysteine 153
A redox-regulated disulphide may form within Mediator of RNA polymerase II transcription subunit 20 between cysteines 75 and 84.

Details

Redox score ?
79
PDB code
6w1s
Structure name
atomic model of the mammalian mediator complex
Structure deposition date
2020-03-04
Thiol separation (Å)
2
Half-sphere exposure sum ?
96
Minimum pKa ?
nan
% buried
nan
Peptide accession
Q9R0X0
Residue number A
75
Residue number B
84
Peptide name
Mediator of RNA polymerase II transcription subunit 20

Ligandability

Cysteine 75 of Mediator of RNA polymerase II transcription subunit 20

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 84 of Mediator of RNA polymerase II transcription subunit 20

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Mediator of RNA polymerase II transcription subunit 20 between cysteines 147 and 153.

Details

Redox score ?
73
PDB code
7nvr
Structure name
human mediator with rna polymerase ii pre-initiation complex
Structure deposition date
2021-03-15
Thiol separation (Å)
4
Half-sphere exposure sum ?
72
Minimum pKa ?
8
% buried
94
Peptide accession
Q9H944
Residue number A
147
Residue number B
153
Peptide name
Mediator of RNA polymerase II transcription subunit 20

Ligandability

Cysteine 147 of Mediator of RNA polymerase II transcription subunit 20

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 153 of Mediator of RNA polymerase II transcription subunit 20

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Mediator of RNA polymerase II transcription subunit 20 between cysteines 5 and 153. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
35
PDB code
7enj
Structure name
human mediator (deletion of med1-idr) in a tail-bent conformation (med-b)
Structure deposition date
2021-04-17
Thiol separation (Å)
10
Half-sphere exposure sum ?
82
Minimum pKa ?
9
% buried
94
Peptide accession
Q9H944
Residue number A
5
Residue number B
153
Peptide name
Mediator of RNA polymerase II transcription subunit 20

Ligandability

Cysteine 5 of Mediator of RNA polymerase II transcription subunit 20

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 153 of Mediator of RNA polymerase II transcription subunit 20

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

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