ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

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DNA-directed RNA polymerase III subunit RPC5

Intermolecular
Cysteine 70 and cysteine 20 of DNA-directed RNA polymerase III subunit RPC10
Cysteine 25 of DNA-directed RNA polymerase III subunit RPC10 and cysteine 70
Intramolecular
Cysteine 692 and cysteine 693
A redox-regulated disulphide may form between cysteine 70 of DNA-directed RNA polymerase III subunit RPC5 and cysteine 20 of DNA-directed RNA polymerase III subunit RPC10. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
43
PDB code
7du2
Structure name
rna polymerase iii ec complex in post-translocation state
Structure deposition date
2021-01-07
Thiol separation (Å)
10
Half-sphere exposure sum ?
49
Minimum pKa ?
8
% buried
24
Peptide A name
DNA-directed RNA polymerase III subunit RPC5
Peptide B name
DNA-directed RNA polymerase III subunit RPC10
Peptide A accession
Q9NVU0
Peptide B accession
Q9Y2Y1
Peptide A residue number
70
Peptide B residue number
20

Ligandability

Cysteine 70 of DNA-directed RNA polymerase III subunit RPC5

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 20 of DNA-directed RNA polymerase III subunit RPC10

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form between cysteine 25 of DNA-directed RNA polymerase III subunit RPC10 and cysteine 70 of DNA-directed RNA polymerase III subunit RPC5. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
43
PDB code
7d58
Structure name
cryo-em structure of human rna polymerase iii in elongating state
Structure deposition date
2020-09-25
Thiol separation (Å)
10
Half-sphere exposure sum ?
59
Minimum pKa ?
8
% buried
nan
Peptide A name
DNA-directed RNA polymerase III subunit RPC10
Peptide B name
DNA-directed RNA polymerase III subunit RPC5
Peptide A accession
Q9Y2Y1
Peptide B accession
Q9NVU0
Peptide A residue number
25
Peptide B residue number
70

Ligandability

Cysteine 25 of DNA-directed RNA polymerase III subunit RPC10

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 70 of DNA-directed RNA polymerase III subunit RPC5

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within DNA-directed RNA polymerase III subunit RPC5 between cysteines 692 and 693. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
54
PDB code
7asv
Structure name
crystal structure of twhd2 of rpc5 subunit of human rna polymerase iii
Structure deposition date
2020-10-28
Thiol separation (Å)
6
Half-sphere exposure sum ?
75
Minimum pKa ?
12
% buried
98
Peptide accession
Q9NVU0
Residue number A
692
Residue number B
693
Peptide name
DNA-directed RNA polymerase III subunit RPC5

Ligandability

Cysteine 692 of DNA-directed RNA polymerase III subunit RPC5

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 693 of DNA-directed RNA polymerase III subunit RPC5

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

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