ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

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DNA polymerase lambda

Intermolecular
Cysteine 543 and cysteine 543
Cysteine 300 and cysteine 300
Intramolecular
Cysteine 412 and cysteine 415
A redox-regulated disulphide may form between two units of DNA polymerase lambda at cysteines 543 and 543.

Details

Redox score ?
81
PDB code
4k4i
Structure name
ternary crystal structures of a human dna polymerase lambda in complex with dna and (-)ftc-tp
Structure deposition date
2013-04-12
Thiol separation (Å)
4
Half-sphere exposure sum ?
35
Minimum pKa ?
9
% buried
0
Peptide A name
DNA polymerase lambda
Peptide B name
DNA polymerase lambda
Peptide A accession
Q9UGP5
Peptide B accession
Q9UGP5
Peptide A residue number
543
Peptide B residue number
543

Ligandability

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form between two units of DNA polymerase lambda at cysteines 300 and 300.

Details

Redox score ?
61
PDB code
7m0e
Structure name
pre-catalytic synaptic complex of dna polymerase lambda with gapped dsb substrate and incoming dumpnpp
Structure deposition date
2021-03-10
Thiol separation (Å)
5
Half-sphere exposure sum ?
62
Minimum pKa ?
11
% buried
64
Peptide A name
DNA polymerase lambda
Peptide B name
DNA polymerase lambda
Peptide A accession
Q9UGP5
Peptide B accession
Q9UGP5
Peptide A residue number
300
Peptide B residue number
300

Ligandability

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within DNA polymerase lambda between cysteines 412 and 415. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
34
PDB code
4fo6
Structure name
crystal structure of the pre-catalytic ternary complex of polymerase lambda with a datp analog opposite a templating t and an rcmp at the primer terminus
Structure deposition date
2012-06-20
Thiol separation (Å)
9
Half-sphere exposure sum ?
64
Minimum pKa ?
12
% buried
97
Peptide accession
Q9UGP5
Residue number A
412
Residue number B
415
Peptide name
DNA polymerase lambda

Ligandability

Cysteine 412 of DNA polymerase lambda

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 415 of DNA polymerase lambda

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

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