ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

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Mitotic spindle assembly checkpoint protein MAD2B

Intermolecular
Cysteine 144 and cysteine 1999 of DNA polymerase zeta catalytic subunit
Cysteine 70 and cysteine 1997 of DNA polymerase zeta catalytic subunit
Intramolecular
Cysteine 20 and cysteine 70
A redox-regulated disulphide may form between cysteine 144 of Mitotic spindle assembly checkpoint protein MAD2B and cysteine 1999 of DNA polymerase zeta catalytic subunit. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
37
PDB code
6bi7
Structure name
crystal structure of rev7-wt/rev3 as a monomer under high-salt conditions
Structure deposition date
2017-11-01
Thiol separation (Å)
10
Half-sphere exposure sum ?
59
Minimum pKa ?
10
% buried
27
Peptide A name
Mitotic spindle assembly checkpoint protein MAD2B
Peptide B name
DNA polymerase zeta catalytic subunit
Peptide A accession
Q9UI95
Peptide B accession
O60673
Peptide A residue number
144
Peptide B residue number
1999

Ligandability

Cysteine 144 of Mitotic spindle assembly checkpoint protein MAD2B

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 1999 of DNA polymerase zeta catalytic subunit

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form between cysteine 70 of Mitotic spindle assembly checkpoint protein MAD2B and cysteine 1997 of DNA polymerase zeta catalytic subunit. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
36
PDB code
6ekm
Structure name
crystal structure of mammalian rev7 in complex with human rev3 second binding site
Structure deposition date
2017-09-26
Thiol separation (Å)
10
Half-sphere exposure sum ?
60
Minimum pKa ?
10
% buried
34
Peptide A name
Mitotic spindle assembly checkpoint protein MAD2B
Peptide B name
DNA polymerase zeta catalytic subunit
Peptide A accession
Q9D752
Peptide B accession
O60673
Peptide A residue number
70
Peptide B residue number
1997

Ligandability

Cysteine 70 of Mitotic spindle assembly checkpoint protein MAD2B

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 1997 of DNA polymerase zeta catalytic subunit

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Mitotic spindle assembly checkpoint protein MAD2B between cysteines 20 and 70. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
34
PDB code
3abe
Structure name
structure of human rev7 in complex with a human rev3 fragment in a tetragonal crystal
Structure deposition date
2009-12-07
Thiol separation (Å)
10
Half-sphere exposure sum ?
65
Minimum pKa ?
10
% buried
72
Peptide accession
Q9UI95
Residue number A
20
Residue number B
70
Peptide name
Mitotic spindle assembly checkpoint protein MAD2B

Ligandability

Cysteine 20 of Mitotic spindle assembly checkpoint protein MAD2B

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 70 of Mitotic spindle assembly checkpoint protein MAD2B

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

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