ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

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Endoribonuclease Dicer

Intermolecular
Cysteine 284 and cysteine 325 of RISC-loading complex subunit TARBP2
Intramolecular
Cysteine 768 and cysteine 877
Cysteine 306 and cysteine 359
A redox-regulated disulphide may form between cysteine 284 of Endoribonuclease Dicer and cysteine 325 of RISC-loading complex subunit TARBP2. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
31
PDB code
5zak
Structure name
cryo-em structure of human dicer and its complexes with a pre-mirna substrate
Structure deposition date
2018-02-07
Thiol separation (Å)
10
Half-sphere exposure sum ?
76
Minimum pKa ?
12
% buried
76
Peptide A name
Endoribonuclease Dicer
Peptide B name
RISC-loading complex subunit TARBP2
Peptide A accession
Q9UPY3
Peptide B accession
Q15633
Peptide A residue number
284
Peptide B residue number
325

Ligandability

Cysteine 284 of Endoribonuclease Dicer

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 325 of RISC-loading complex subunit TARBP2

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Endoribonuclease Dicer between cysteines 768 and 877 (758 and 867 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
41
PDB code
4ngf
Structure name
structure of human dicer platform-paz-connector helix cassette in complex with 17-mer sirna having 5'-p and uu-3' ends (3
Structure deposition date
2013-11-01
Thiol separation (Å)
10
Half-sphere exposure sum ?
54
Minimum pKa ?
9
% buried
29
Peptide accession
Q9UPY3
Residue number A
768
Residue number B
877
Peptide name
Endoribonuclease Dicer

Ligandability

Cysteine 768 of Endoribonuclease Dicer

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 877 of Endoribonuclease Dicer

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form within Endoribonuclease Dicer between cysteines 306 and 359 (308 and 361 respectively in this structure). However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
32
PDB code
4wyq
Structure name
crystal structure of the dicer-trbp interface
Structure deposition date
2014-11-18
Thiol separation (Å)
10
Half-sphere exposure sum ?
73
Minimum pKa ?
12
% buried
76
Peptide accession
Q9UPY3
Residue number A
306
Residue number B
359
Peptide name
Endoribonuclease Dicer

Ligandability

Cysteine 306 of Endoribonuclease Dicer

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

Cysteine 359 of Endoribonuclease Dicer

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

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