ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

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Mitotic spindle assembly checkpoint protein MAD1

Intermolecular
Cysteine 633 and cysteine 633
Cysteine 568 and cysteine 568
Cysteine 662 and cysteine 662
A redox-regulated disulphide may form between two units of Mitotic spindle assembly checkpoint protein MAD1 at cysteines 633 and 633.

Details

Redox score ?
66
PDB code
7b1f
Structure name
orthorhombic p212121 structure of human mad1 c-terminal domain in complex with phosphorylated bub1 cd1 domain
Structure deposition date
2020-11-24
Thiol separation (Å)
5
Half-sphere exposure sum ?
83
Minimum pKa ?
8
% buried
100
Peptide A name
Mitotic spindle assembly checkpoint protein MAD1
Peptide B name
Mitotic spindle assembly checkpoint protein MAD1
Peptide A accession
Q9Y6D9
Peptide B accession
Q9Y6D9
Peptide A residue number
633
Peptide B residue number
633

Ligandability

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form between two units of Mitotic spindle assembly checkpoint protein MAD1 at cysteines 568 and 568. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
56
PDB code
1go4
Structure name
crystal structure of mad1-mad2 reveals a conserved mad2 binding motif in mad1 and cdc20
Structure deposition date
2001-10-17
Thiol separation (Å)
7
Half-sphere exposure sum ?
38
Minimum pKa ?
9
% buried
0
Peptide A name
Mitotic spindle assembly checkpoint protein MAD1
Peptide B name
Mitotic spindle assembly checkpoint protein MAD1
Peptide A accession
Q9Y6D9
Peptide B accession
Q9Y6D9
Peptide A residue number
568
Peptide B residue number
568

Ligandability

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

A redox-regulated disulphide may form between two units of Mitotic spindle assembly checkpoint protein MAD1 at cysteines 662 and 662. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
34
PDB code
7b1h
Structure name
monoclinic p21 structure of human mad1 c-terminal domain in complex with phosphorylated bub1 cd1 domain
Structure deposition date
2020-11-24
Thiol separation (Å)
9
Half-sphere exposure sum ?
66
Minimum pKa ?
11
% buried
80
Peptide A name
Mitotic spindle assembly checkpoint protein MAD1
Peptide B name
Mitotic spindle assembly checkpoint protein MAD1
Peptide A accession
Q9Y6D9
Peptide B accession
Q9Y6D9
Peptide A residue number
662
Peptide B residue number
662

Ligandability

Not ligandable in the dataset of Yang et al.

Not ligandable in the dataset of Yan et al.

Not ligandable in the dataset of Backus et al.

Not ligandable in the dataset of Vinogradova et al.

Not ligandable in the dataset of Cao et al.

Not ligandable in the dataset of Kuljanin et al.

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